Fish Oil - Omega-3 Exerts Protective Effect in Oxidative Stress and Liver Dysfunctions Resulting from Experimental Sepsis.

Autor: Velasque MJSG; Graduate Program in Pathology - Laboratory of Computational, Molecular, and Cellular Biophysics, Universidade Federal de Ciências da Saúde de Porto Alegre (UFCSPA), Brazil., Branchini G; Graduate Program in Pathology - Laboratory of Computational, Molecular, and Cellular Biophysics, Universidade Federal de Ciências da Saúde de Porto Alegre (UFCSPA), Brazil., Catarina AV; Graduate Program in Pathology - Laboratory of Computational, Molecular, and Cellular Biophysics, Universidade Federal de Ciências da Saúde de Porto Alegre (UFCSPA), Brazil., Bettoni L; Graduate Program in Pathology - Laboratory of Computational, Molecular, and Cellular Biophysics, Universidade Federal de Ciências da Saúde de Porto Alegre (UFCSPA), Brazil., Fernandes RS; Graduate Program in Health Sciences - Laboratory of Translational Physiology - Universidade Federal de Ciências da Saúde de Porto Alegre (UFCSPA), Brazil., Da Silva AF; Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Brazil., Dorneles GP; Laboratory of Cellular and Molecular Immunology - Universidade Federal de Ciências da Saúde de Porto Alegre (UFCSPA), Brazil., da Silva IM; Laboratory of Cellular and Molecular Immunology - Universidade Federal de Ciências da Saúde de Porto Alegre (UFCSPA), Brazil., Santos MA; Laboratory of Cellular and Molecular Immunology - Universidade Federal de Ciências da Saúde de Porto Alegre (UFCSPA), Brazil., Sumienski J; Laboratory of Immunology and Microbiology - Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Brazil., Peres A; Laboratory of Cellular and Molecular Immunology - Universidade Federal de Ciências da Saúde de Porto Alegre (UFCSPA), Brazil., Roehe AV; Graduate Program in Pathology - Laboratory of Pathology - Universidade Federal de Ciências da Saúde de Porto Alegre (UFCSPA), Brazil., Kohek MBDF; Laboratory of Cellular and Molecular Immunology - Universidade Federal de Ciências da Saúde de Porto Alegre (UFCSPA), Brazil., Porawski M; Laboratory of Behavioral and Metabolic Physiology - Universidade Federal de Ciências da Saúde de Porto Alegre (UFCSPA), Brazil., Nunes FB; Graduate Program in Pathology - Laboratory of Computational, Molecular, and Cellular Biophysics, Universidade Federal de Ciências da Saúde de Porto Alegre (UFCSPA), Brazil.; Laboratory of Inflammation and Cellular Biophysics - Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Brazil.
Jazyk: angličtina
Zdroj: Journal of clinical and experimental hepatology [J Clin Exp Hepatol] 2023 Jan-Feb; Vol. 13 (1), pp. 64-74. Date of Electronic Publication: 2022 Jul 10.
DOI: 10.1016/j.jceh.2022.07.001
Abstrakt: Background: Sepsis is a severe global health problem, with high morbidity and mortality. In sepsis, one of the main affected organs is the liver. Hepatic alterations characterize a negative prognostic. Omega-3 fatty acids (ω3), eicosapentaenoic acid, and docosahexaenoic acid, are part of the main families of polyunsaturated fatty acids. ω3 has been used in studies as sepsis treatment and as a treatment for non-alcoholic liver disease.
Aim: We aimed to evaluate the effects of treatment with fish oil (FO) rich in ω3 on liver changes and damage resulting from experimental sepsis.
Methodology: A model of severe sepsis in Wistar rats was used. Oxidative stress in the liver tissue was evaluated by means of tests of thiobarbituric acid reactive substances, 2,7-dihydrodichlorofluorescein diacetate , catalase, and glutathione peroxidase, in the serum TBARS, DCF, thiols and, to assess liver dysfunction, alanine aminotransferase and aspartate aminotransferase. Hepatic tissue damage was evaluated using H&E histology.
Results: In assessments of oxidative stress in liver tissue, a protective effect was observed in the tests of TBARS, DCF, CAT, and GPx, when compared the sepsis versus sepsis+ω3 groups. Regarding the oxidative stress in serum, a protective effect of treatment with ω3 was observed in the TBARS, DCF, and thiols assays, in the comparison between the sepsis and sepsis+ω3 groups. ω3 had also a beneficial effect on biochemical parameters in serum in the analysis of ALT, creatinine, urea, and lactate, observed in the comparison between the sepsis and sepsis+ω3 groups.
Conclusion: The results suggest ω3 as a liver protector during sepsis with an antioxidant effect, alleviating injuries and dysfunctions.
(© 2022 Indian National Association for Study of the Liver. Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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