Urinary Metabolome Analysis Reveals Potential Microbiota Alteration and Electrophilic Burden Induced by High Red Meat Diet: Results from the French NutriNet-Santé Cohort and an In Vivo Intervention Study in Rats.

Autor: Mervant L; Toxalim, Toulouse University, INRAE, ENVT, INP-Purpan, UPS, Toulouse, 31027, France.; MetaboHUB-MetaToul, National Infrastructure of Metabolomics and Fluxomics, Toulouse, 31077, France.; French Network for Nutrition and Cancer Research (NACRe Network), Jouy-en-Josas, 78352, France., Tremblay-Franco M; Toxalim, Toulouse University, INRAE, ENVT, INP-Purpan, UPS, Toulouse, 31027, France.; MetaboHUB-MetaToul, National Infrastructure of Metabolomics and Fluxomics, Toulouse, 31077, France., Olier M; Toxalim, Toulouse University, INRAE, ENVT, INP-Purpan, UPS, Toulouse, 31027, France., Jamin E; Toxalim, Toulouse University, INRAE, ENVT, INP-Purpan, UPS, Toulouse, 31027, France.; MetaboHUB-MetaToul, National Infrastructure of Metabolomics and Fluxomics, Toulouse, 31077, France., Martin JF; Toxalim, Toulouse University, INRAE, ENVT, INP-Purpan, UPS, Toulouse, 31027, France.; MetaboHUB-MetaToul, National Infrastructure of Metabolomics and Fluxomics, Toulouse, 31077, France., Trouilh L; Plateforme Genome et Transcriptome (GeT-Biopuces), Toulouse Biotechnology Institute (TBI), Université ide Toulouse, CNRS, INRAE, INSA, 135 avenue de Rangueil, Toulouse, F-31077, France., Buisson C; Toxalim, Toulouse University, INRAE, ENVT, INP-Purpan, UPS, Toulouse, 31027, France.; French Network for Nutrition and Cancer Research (NACRe Network), Jouy-en-Josas, 78352, France., Naud N; Toxalim, Toulouse University, INRAE, ENVT, INP-Purpan, UPS, Toulouse, 31027, France.; French Network for Nutrition and Cancer Research (NACRe Network), Jouy-en-Josas, 78352, France., Maslo C; Toxalim, Toulouse University, INRAE, ENVT, INP-Purpan, UPS, Toulouse, 31027, France., Héliès-Toussaint C; Toxalim, Toulouse University, INRAE, ENVT, INP-Purpan, UPS, Toulouse, 31027, France.; French Network for Nutrition and Cancer Research (NACRe Network), Jouy-en-Josas, 78352, France., Fouché E; Toxalim, Toulouse University, INRAE, ENVT, INP-Purpan, UPS, Toulouse, 31027, France.; French Network for Nutrition and Cancer Research (NACRe Network), Jouy-en-Josas, 78352, France., Kesse-Guyot E; French Network for Nutrition and Cancer Research (NACRe Network), Jouy-en-Josas, 78352, France.; Sorbonne Paris Nord University, INSERM U1153, INRAe U1125, CNAM, Nutritional Epidemiology Research Team (EREN), Epidemiology and Statistics Research Center - University of Paris (CRESS), 74 rue Marcel Cachin, Bobigny, 93017, France., Hercberg S; French Network for Nutrition and Cancer Research (NACRe Network), Jouy-en-Josas, 78352, France.; Sorbonne Paris Nord University, INSERM U1153, INRAe U1125, CNAM, Nutritional Epidemiology Research Team (EREN), Epidemiology and Statistics Research Center - University of Paris (CRESS), 74 rue Marcel Cachin, Bobigny, 93017, France., Galan P; Sorbonne Paris Nord University, INSERM U1153, INRAe U1125, CNAM, Nutritional Epidemiology Research Team (EREN), Epidemiology and Statistics Research Center - University of Paris (CRESS), 74 rue Marcel Cachin, Bobigny, 93017, France., Deschasaux-Tanguy M; French Network for Nutrition and Cancer Research (NACRe Network), Jouy-en-Josas, 78352, France.; Sorbonne Paris Nord University, INSERM U1153, INRAe U1125, CNAM, Nutritional Epidemiology Research Team (EREN), Epidemiology and Statistics Research Center - University of Paris (CRESS), 74 rue Marcel Cachin, Bobigny, 93017, France., Touvier M; French Network for Nutrition and Cancer Research (NACRe Network), Jouy-en-Josas, 78352, France.; Sorbonne Paris Nord University, INSERM U1153, INRAe U1125, CNAM, Nutritional Epidemiology Research Team (EREN), Epidemiology and Statistics Research Center - University of Paris (CRESS), 74 rue Marcel Cachin, Bobigny, 93017, France., Pierre F; Toxalim, Toulouse University, INRAE, ENVT, INP-Purpan, UPS, Toulouse, 31027, France.; French Network for Nutrition and Cancer Research (NACRe Network), Jouy-en-Josas, 78352, France., Debrauwer L; Toxalim, Toulouse University, INRAE, ENVT, INP-Purpan, UPS, Toulouse, 31027, France.; MetaboHUB-MetaToul, National Infrastructure of Metabolomics and Fluxomics, Toulouse, 31077, France., Guéraud F; Toxalim, Toulouse University, INRAE, ENVT, INP-Purpan, UPS, Toulouse, 31027, France.; French Network for Nutrition and Cancer Research (NACRe Network), Jouy-en-Josas, 78352, France.
Jazyk: angličtina
Zdroj: Molecular nutrition & food research [Mol Nutr Food Res] 2023 Mar; Vol. 67 (5), pp. e2200432. Date of Electronic Publication: 2023 Feb 02.
DOI: 10.1002/mnfr.202200432
Abstrakt: Scope: High red and processed meat consumption is associated with several adverse outcomes such as colorectal cancer and overall global mortality. However, the underlying mechanisms remain debated and need to be elucidated.
Methods and Results: Urinary untargeted Liquid Chromatography-Mass Spectrometry (LC-MS) metabolomics data from 240 subjects from the French cohort NutriNet-Santé are analyzed. Individuals are matched and divided into three groups according to their consumption of red and processed meat: high red and processed meat consumers, non-red and processed meat consumers, and at random group. Results are supported by a preclinical experiment where rats are fed either a high red meat or a control diet. Microbiota derived metabolites, in particular indoxyl sulfate and cinnamoylglycine, are found impacted by the high red meat diet in both studies, suggesting a modification of microbiota by the high red/processed meat diet. Rat microbiota sequencing analysis strengthens this observation. Although not evidenced in the human study, rat mercapturic acid profile concomitantly reveals an increased lipid peroxidation induced by high red meat diet.
Conclusion: Novel microbiota metabolites are identified as red meat consumption potential biomarkers, suggesting a deleterious effect, which could partly explain the adverse effects associated with high red and processed meat consumption.
(© 2023 The Authors. Molecular Nutrition & Food Research published by Wiley-VCH GmbH.)
Databáze: MEDLINE
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