Semi-automated water sampling module for repeated sampling and concentration of Bacillus cereus group spores.

Autor: Hassen WM; Laboratory for Quantum Semiconductors and Photon-based BioNanotechnology, Department of Electrical and Computer Engineering, Interdisciplinary Institute for Technological Innovation (3IT), CNRS IRL-3463, Université de Sherbrooke, 3000, boul. de l'Université, Sherbrooke, QC, J1K 0A5, Canada., Vermette J; Laboratory for Quantum Semiconductors and Photon-based BioNanotechnology, Department of Electrical and Computer Engineering, Interdisciplinary Institute for Technological Innovation (3IT), CNRS IRL-3463, Université de Sherbrooke, 3000, boul. de l'Université, Sherbrooke, QC, J1K 0A5, Canada., Moteshareie H; Laboratory for Quantum Semiconductors and Photon-based BioNanotechnology, Department of Electrical and Computer Engineering, Interdisciplinary Institute for Technological Innovation (3IT), CNRS IRL-3463, Université de Sherbrooke, 3000, boul. de l'Université, Sherbrooke, QC, J1K 0A5, Canada.; Biotechnology Laboratory, Environmental Health Science and Research Bureau, Healthy Environments and Consumer Safety Branch, Health Canada, 251 Sir Frederick Banting Driveway, Ottawa, ON, K1A 0K9, Canada., Tayabali AF; Laboratory for Quantum Semiconductors and Photon-based BioNanotechnology, Department of Electrical and Computer Engineering, Interdisciplinary Institute for Technological Innovation (3IT), CNRS IRL-3463, Université de Sherbrooke, 3000, boul. de l'Université, Sherbrooke, QC, J1K 0A5, Canada.; Biotechnology Laboratory, Environmental Health Science and Research Bureau, Healthy Environments and Consumer Safety Branch, Health Canada, 251 Sir Frederick Banting Driveway, Ottawa, ON, K1A 0K9, Canada., Dubowski JJ; Laboratory for Quantum Semiconductors and Photon-based BioNanotechnology, Department of Electrical and Computer Engineering, Interdisciplinary Institute for Technological Innovation (3IT), CNRS IRL-3463, Université de Sherbrooke, 3000, boul. de l'Université, Sherbrooke, QC, J1K 0A5, Canada. jan.j.dubowski@usherbrooke.ca.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2023 Jan 16; Vol. 13 (1), pp. 831. Date of Electronic Publication: 2023 Jan 16.
DOI: 10.1038/s41598-023-27900-0
Abstrakt: Monitoring the presence of pathogenic Bacillus spores is important for industrial applications, as well as necessary for ensuring human health. Bacillus thuringiensis is used as a biopesticide against several insect pests. Bacillus cereus spores are a significant cause of food poisoning, and Bacillus anthracis is a recognized biosecurity threat. Laboratory-based methods, such as polymerase chain reaction, enzyme-linked immunosorbent assay, or matrix-assisted laser desorption ionization spectroscopy provide sensitive detection of bacteria and spores, but the application of those methods for quasi-continuous environmental monitoring presents a significant challenge requiring frequent human intervention. To address this challenge, we developed a workstation for quasi-autonomous monitoring of water reservoirs for the presence of bacteria and spores, and designed and validated the functionality of a microprocessor-controlled module capable of repetitive collection and pre-concentration of spores in liquid samples tested with fiberglass (FG), polyether sulfone and polyvinylidene fluoride filters. The best results were obtained with FG filters delivering a 20× concentration of B. thuringiensis and B. cereus spores from saline suspensions. The successful 20× pre-concentration of Bacillus spores demonstrated with FG filters could be repeated up to 3 times when bleach decontamination is applied between filtrations. Taken together, our results demonstrate an attractive instrument suitable for semi-automated, quasi-continuous sampling and pre-processing of water samples for biosensing of bacterial spores originating from a complex environment.
(© 2023. Crown.)
Databáze: MEDLINE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje