Mitigating the allergenicity of peanut allergen Ara h 1 by cold atmospheric pressure argon plasma jet.

Autor: Hsu FC; Department of Horticulture and Landscape Architecture, National Taiwan University, Taipei, Taiwan., Lin WT; Institute of Food Science and Technology, National Taiwan University, Taipei, Taiwan., Hsieh KC; Institute of Food Science and Technology, National Taiwan University, Taipei, Taiwan., Cheng KC; Institute of Food Science and Technology, National Taiwan University, Taipei, Taiwan.; Institute of Biotechnology, College of Bioresources and Agriculture, National Taiwan University, Taipei, Taiwan.; Department of Optometry, Asia University, Taichung, Taiwan.; Department of Medical Research, China Medical University Hospital, China Medical University, Taichung, Taiwan., Wu JS; Institute of Food Science and Technology, National Taiwan University, Taipei, Taiwan., Ting Y; Institute of Food Science and Technology, National Taiwan University, Taipei, Taiwan.
Jazyk: angličtina
Zdroj: Journal of the science of food and agriculture [J Sci Food Agric] 2023 Apr; Vol. 103 (6), pp. 3017-3027. Date of Electronic Publication: 2023 Feb 11.
DOI: 10.1002/jsfa.12454
Abstrakt: Background: Peanut allergy is recognized as a major food allergy that triggers severe and even fatal symptoms. Avoidance of peanuts in the diet is the main option for current safety management. Processing techniques reducing peanut allergenicity are required to develop other options. Cold plasma is currently considered as a novel non-thermal approach to alter protein structure and has the potential to alleviate immunoreactivity of protein allergen.
Results: The application of a cold argon plasma jet to peanut protein extract could reduce the amount of a 64 kDa protein band corresponding to a major peanut allergen Ara h 1 using sodium dodecyl sulfate-polyacrylamide gel electrophoresis, but the overall protein size distribution did not change significantly. A decrease in peanut protein solubility was a possible cause that led to the loss of protein content in the soluble fraction. Immunoblotting and enzyme-linked immunosorbent assay elucidated that the immunoreactivity of Ara h 1 was significantly decreased with the time treated with plasma. Ara h 1 antigenicity reduced by 38% after five scans (approximately 3 min) of cold argon plasma jet treatment, and the reduction was up to 66% after approximately 15 min of treatment.
Conclusion: The results indicate that cold argon plasma jet treatment could be a suitable platform for alleviating the immunoreactivity of peanut protein. This work demonstrates an efficient, compact, and rapid platform for mitigating the allergenicity of peanuts, and shows great potential for the plasma platform as a non-thermal technique in the food industry. © 2023 Society of Chemical Industry.
(© 2023 Society of Chemical Industry.)
Databáze: MEDLINE
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