Breakfast partly restores the anti-inflammatory function of high-density lipoproteins from patients with type 2 diabetes mellitus.
Autor: | Lemmers RFH; Department of Internal Medicine, Section Pharmacology and Vascular Medicine, Erasmus University Medical Center, Rotterdam, the Netherlands.; Máxima Medical Center, Internal Medicine, Eindhoven, the Netherlands., Martens NEMA; Department of Internal Medicine, Section Pharmacology and Vascular Medicine, Erasmus University Medical Center, Rotterdam, the Netherlands., Maas AH; Máxima Medical Center, Internal Medicine, Eindhoven, the Netherlands., van Vark-van der Zee LC; Department of Internal Medicine, Section Pharmacology and Vascular Medicine, Erasmus University Medical Center, Rotterdam, the Netherlands., Leijten FPJ; Department of Internal Medicine, Section Pharmacology and Vascular Medicine, Erasmus University Medical Center, Rotterdam, the Netherlands., Groot-van Ruijven CM; Department of Immunology, Erasmus University Medical Center, Rotterdam, the Netherlands., van Hoek M; Department of Internal Medicine, Section Pharmacology and Vascular Medicine, Erasmus University Medical Center, Rotterdam, the Netherlands., Lieverse AG; Máxima Medical Center, Internal Medicine, Eindhoven, the Netherlands., Sijbrands EJG; Department of Internal Medicine, Section Pharmacology and Vascular Medicine, Erasmus University Medical Center, Rotterdam, the Netherlands., Haak HR; Máxima Medical Center, Internal Medicine, Eindhoven, the Netherlands., Leenen PJM; Department of Immunology, Erasmus University Medical Center, Rotterdam, the Netherlands., Verhoeven AJM; Department of Internal Medicine, Section Pharmacology and Vascular Medicine, Erasmus University Medical Center, Rotterdam, the Netherlands., Dik WA; Department of Immunology, Erasmus University Medical Center, Rotterdam, the Netherlands.; Laboratory Medical Immunology, Department of Immunology, Erasmus University Medical Center, Rotterdam, the Netherlands., Mulder MT; Department of Internal Medicine, Section Pharmacology and Vascular Medicine, Erasmus University Medical Center, Rotterdam, the Netherlands. |
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Jazyk: | angličtina |
Zdroj: | Atherosclerosis plus [Atheroscler Plus] 2021 Aug 24; Vol. 44, pp. 43-50. Date of Electronic Publication: 2021 Aug 24 (Print Publication: 2021). |
DOI: | 10.1016/j.athplu.2021.08.006 |
Abstrakt: | Background and Aims: High-density lipoproteins (HDL) of patients with type 2 diabetes mellitus (T2DM) have impaired anti-inflammatory activities. The anti-inflammatory activity of HDL has been determined ex vivo after isolation by different methods from blood mostly obtained after overnight fasting. We first determined the effect of the HDL isolation method, and subsequently the effect of food intake on the anti-inflammatory function of HDL from T2DM patients. Methods: Blood was collected from healthy controls and T2DM patients after an overnight fast, and from T2DM patients 3 h after breakfast ( n = 17 each). HDL was isolated by a two-step density gradient ultracentrifugation in iodixanol (HDL Results: HDL isolated by the three different methods from healthy controls inhibited TNFα-induced VCAM-1 expression in HCAEC. With apoA-I at 0.7 μM, HDL Conclusions: The ex vivo anti-inflammatory activity of HDL is affected by the HDL isolation method. Two-step ultracentrifugation in an iodixanol gradient is a suitable method for HDL isolation when testing HDL anti-inflammatory function. The anti-inflammatory activity of HDL from overnight fasted T2DM patients is significantly impaired in REC but not in HCAEC. The anti-inflammatory function of HDL is partly restored by food intake. Competing Interests: The authors declare that they have no conflict of interest. (© 2021 The Authors.) |
Databáze: | MEDLINE |
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