Polymorphism, Nanostructures, and Barrier Properties of Ceramide-Based Lipid Films.

Autor:	Pullmannová P; Skin Barrier Research Group, Charles University, Faculty of Pharmacy in Hradec Králové, Heyrovského 1203, 500 05Hradec Králové, Czech Republic., Čuříková-Kindlová BA; Faculty of Chemical Technology, University of Chemistry and Technology Prague, Technická 5, 166 28Prague, Czech Republic., Ondrejčeková V; Skin Barrier Research Group, Charles University, Faculty of Pharmacy in Hradec Králové, Heyrovského 1203, 500 05Hradec Králové, Czech Republic., Kováčik A; Skin Barrier Research Group, Charles University, Faculty of Pharmacy in Hradec Králové, Heyrovského 1203, 500 05Hradec Králové, Czech Republic., Dvořáková K; Faculty of Chemical Technology, University of Chemistry and Technology Prague, Technická 5, 166 28Prague, Czech Republic., Dulanská L; Skin Barrier Research Group, Charles University, Faculty of Pharmacy in Hradec Králové, Heyrovského 1203, 500 05Hradec Králové, Czech Republic., Georgii R; Heinz Maier-Leibnitz Zentrum (MLZ), Technische Universität München, Lichtenbergstr. 1, 85748Garching, Germany., Majcher A; Skin Barrier Research Group, Charles University, Faculty of Pharmacy in Hradec Králové, Heyrovského 1203, 500 05Hradec Králové, Czech Republic., Maixner J; Faculty of Chemical Technology, University of Chemistry and Technology Prague, Technická 5, 166 28Prague, Czech Republic., Kučerka N; Faculty of Pharmacy, Comenius University Bratislava, Odbojárov 10, 832 32Bratislava, Slovakia.; Frank Laboratory of Neutron Physics, Joint Institute for Nuclear Research, Joliot-Curie 6, 141980Dubna, Russia., Zbytovská J; Skin Barrier Research Group, Charles University, Faculty of Pharmacy in Hradec Králové, Heyrovského 1203, 500 05Hradec Králové, Czech Republic.; Faculty of Chemical Technology, University of Chemistry and Technology Prague, Technická 5, 166 28Prague, Czech Republic., Vávrová K; Skin Barrier Research Group, Charles University, Faculty of Pharmacy in Hradec Králové, Heyrovského 1203, 500 05Hradec Králové, Czech Republic.
Jazyk:	angličtina
Zdroj:	ACS omega [ACS Omega] 2022 Dec 23; Vol. 8 (1), pp. 422-435. Date of Electronic Publication: 2022 Dec 23 (Print Publication: 2023).
DOI:	10.1021/acsomega.2c04924
Abstrakt:	Ceramides belong to sphingolipids, an important group of cellular and extracellular lipids. Their physiological functions range from cell signaling to participation in the formation of barriers against water evaporation. In the skin, they are essential for the permeability barrier, together with free fatty acids and cholesterol. We examined the periodical structure and permeability of lipid films composed of ceramides (Cer; namely, N -lignoceroyl 6-hydroxysphingosine, CerNH24, and N -lignoceroyl sphingosine, CerNS24), lignoceric acid (LIG; 24:0), and cholesterol (Chol). X-ray diffraction experiments showed that the CerNH24-based samples form either a short lamellar phase (SLP, d ∼ 5.4 nm) or a medium lamellar phase (MLP, d = 10.63-10.78 nm) depending on the annealing conditions. The proposed molecular arrangement of the MLP based on extended Cer molecules also agreed with the relative neutron scattering length density profiles obtained from the neutron diffraction data. The presence of MLP increased the lipid film permeability to the lipophilic model permeant (indomethacin) relative to the CerNS24-based control samples and the samples that had the same lipid composition but formed an SLP. Thus, the arrangement of lipids in various nanostructures is responsive to external conditions during sample preparation. This polymorphic behavior directly affects the barrier properties, which could also be (patho)physiologically relevant. Competing Interests: The authors declare no competing financial interest. (© 2022 The Authors. Published by American Chemical Society.)
Databáze:	MEDLINE
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