Engineering a Yellow Thermostable Fluorescent Protein by Rational Design.

Autor:	Anderson MR; Department of Chemistry and Biochemistry, Missouri State University, 901 South National Ave, Springfield, Missouri65897, United States., Padgett CM; Department of Chemistry and Biochemistry, Missouri State University, 901 South National Ave, Springfield, Missouri65897, United States., Dargatz CJ; Department of Chemistry and Biochemistry, Missouri State University, 901 South National Ave, Springfield, Missouri65897, United States., Nichols CR; Department of Chemistry and Biochemistry, Missouri State University, 901 South National Ave, Springfield, Missouri65897, United States., Vittalam KR; Department of Chemistry and Biochemistry, Missouri State University, 901 South National Ave, Springfield, Missouri65897, United States.; Greenwood Laboratory School, 1024 E. Harrison, Springfield, Missouri65897, United States., DeVore NM; Department of Chemistry and Biochemistry, Missouri State University, 901 South National Ave, Springfield, Missouri65897, United States.
Jazyk:	angličtina
Zdroj:	ACS omega [ACS Omega] 2022 Dec 28; Vol. 8 (1), pp. 436-443. Date of Electronic Publication: 2022 Dec 28 (Print Publication: 2023).
DOI:	10.1021/acsomega.2c05005
Abstrakt:	Thermal green protein (TGP) is an extremely stable, highly soluble synthetic green fluorescent protein. The quantum yield of TGP is lower than the closest related natural fluorescent protein, monomeric Azami-Green. We improved the thermal recovery of TGP through the introduction of a chromophore mutation, Q66E. Furthermore, we developed a yellow thermal protein (YTP) via mutation of histidine 193 to tyrosine. Incorporation of Q66E into YTP (YTP-E) improved chemostability and pH stability. Both YTP and YTP-E have superior thermostability compared to TGP or TGP-E. These proteins offer a new option for green or yellow fluorescence under harsh chemical or thermal conditions. Competing Interests: The authors declare no competing financial interest. (© 2022 The Authors. Published by American Chemical Society.)
Databáze:	MEDLINE
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