Effect of a karst system (France) on extended spectrum beta-lactamase (ESBL)-producing Escherichia coli.

Autor: Henriot CP; Chrono-Environnement UMR 6249, CNRS, Université de Franche-Comté, Besançon 25000, France. Electronic address: charles.henriot@univ-fcomte.fr., Celle H; Chrono-Environnement UMR 6249, CNRS, Université de Franche-Comté, Besançon 25000, France., Klaba V; Chrono-Environnement UMR 6249, CNRS, Université de Franche-Comté, Besançon 25000, France., Biguenet A; Hygiène Hospitalière, Centre Hospitalier Universitaire de Besançon, 3 Boulevard Fleming, Besançon 25030, France., Miège C; INRAE, RiverLy, 5 rue de la Doua, CS20244, Villeurbanne 69625, France., Daval A; INRAE, RiverLy, 5 rue de la Doua, CS20244, Villeurbanne 69625, France., Amiotte-Suchet P; UMR CNRS 6282 Biogéosciences, Université de Bourgogne Franche-Comté, 6 Boulevard Gabriel, Dijon 21000, France., Beugnot JC; UMR CNRS 6174 FEMTO-ST, Université de Bourgogne Franche-Comté, 15B Avenue des Montboucons, Besançon 25030, France., Karbowiak T; Institut Agro Dijon, University Bourgogne Franche-Comté, UMR PAM 02 102, 1 Esplanade Erasme, Dijon 21000, France., Bertrand X; Chrono-Environnement UMR 6249, CNRS, Université de Franche-Comté, Besançon 25000, France; Hygiène Hospitalière, Centre Hospitalier Universitaire de Besançon, 3 Boulevard Fleming, Besançon 25030, France.
Jazyk: angličtina
Zdroj: Water research [Water Res] 2023 Feb 15; Vol. 230, pp. 119582. Date of Electronic Publication: 2023 Jan 06.
DOI: 10.1016/j.watres.2023.119582
Abstrakt: Karst aquifers are an important water resource worldwide particularly exposed to anthropogenic pollution, including antibiotic-resistance. The release of antibiotic-resistant bacterial pathogens in the environment is a major public health challenge worldwide. In this One Health study, we aimed to determine the effect of karst on antibiotic-resistant bacteria. For this purpose, we determined the concentrations of extended-spectrum β-lactamases-producing Escherichia coli (ESBL-Ec) for 92 weeks in a rural karst hydrosystem providing drinking water. ESBL-Ec isolates (n = 130) were sequenced by whole genome sequencing. We analysed the isolates at different levels of granularity, i.e., phylogroup, sequence type, presence of antibiotic-resistance genes, mutations conferring antibiotic-resistance, and virulence genes. The ESBL-Ec concentrations were spatially and temporally heterogeneous in the studied karst hydrosystem. ESBL-Ec isolates survived in the karst and their concentrations were mostly explained by the hydrodynamic of the hydrosystem. We demonstrate that the studied karst has no filtration effect on ESBL-Ec, either quantitatively (i.e., in the ESBL-Ec concentrations) or qualitatively (i.e., in the genetic characteristics of ESBL-Ec isolates).
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023. Published by Elsevier Ltd.)
Databáze: MEDLINE
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