Antimicrobial photodynamic therapy mediated by methylene blue-loaded polymeric micelles against Streptococcus mutans and Candida albicans biofilms.

Autor: Soares JCM; School of Pharmaceutical Science, Sao Paulo State University (UNESP), Araraquara, Sao Paulo 14800-903, Brazil., Luiz MT; School of Pharmaceutical Science, Sao Paulo State University (UNESP), Araraquara, Sao Paulo 14800-903, Brazil. Electronic address: marcelatavaresf@hotmail.com., Oshiro Junior JA; School of Pharmaceutical Science, Sao Paulo State University (UNESP), Araraquara, Sao Paulo 14800-903, Brazil., Besegato JF; Departament of Restorative Dentistry, School of Dentistry, Sao Paulo State University (UNESP), Araraquara, Sao Paulo, Brazil., de Melo PBG; Departament of Restorative Dentistry, School of Dentistry, Sao Paulo State University (UNESP), Araraquara, Sao Paulo, Brazil., Rastelli ANS; Departament of Restorative Dentistry, School of Dentistry, Sao Paulo State University (UNESP), Araraquara, Sao Paulo, Brazil., Chorilli M; School of Pharmaceutical Science, Sao Paulo State University (UNESP), Araraquara, Sao Paulo 14800-903, Brazil. Electronic address: marlus.chorilli@unesp.br.
Jazyk: angličtina
Zdroj: Photodiagnosis and photodynamic therapy [Photodiagnosis Photodyn Ther] 2023 Mar; Vol. 41, pp. 103285. Date of Electronic Publication: 2023 Jan 11.
DOI: 10.1016/j.pdpdt.2023.103285
Abstrakt: Background: Streptococcus mutans and Candida albicans can colonize the teeth, the oral cavity as biofilm and can cause oral infections. Thus, strategies to prevent and control oral biofilms are requested. The present study aims the development and characterization of methylene blue (MB)-loaded polymeric micelles for antimicrobial photodynamic therapy (aPDT) against Streptococcus mutans and Candida albicans biofilms METHODS: MB-loaded polymeric micelles were produced and characterized by particle size, polydispersity index, morphology, zeta potential, stability, MB release profile, and antimicrobial effect against S. mutans and C. albicans biofilms.
Results: MB-loaded polymeric micelles showed a reduced particle size, moderate polydisperse profile, spherical and neutral shape, which demonstrated to be promising features to allow micelles penetration into biofilms. Antimicrobial effect against bacterial and yeast biofilms was demonstrated once MB was irradiated by light under 660 nm (aPDT). Furthermore, MB-loaded polymeric micelles showed significant inhibition of S. mutans and C. albicans biofilms. Furthermore, the treatment with MB-micelles incubated with high pre-incubation times (15 and 30 min) were more effective than 5 min. It can be explained by the time required for this nanosystem to penetrate the innermost layer of biofilms and release MB for aPDT.
Conclusion: MB-loaded polymeric micelles can effectively decrease the bacteria and yeast viability and it may cause positive impacts in the clinical practice. Thus, the developed formulation showed potential in the treatment to remove oral biofilms, but clinical studies are needed to confirm its potential.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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