Validation of the slaughterhouse porcine heart model for ex-situ heart perfusion studies.

Autor:	Kaffka Genaamd Dengler SE; Department of Cardiothoracic Surgery, University Medical Center Utrecht, Utrecht, The Netherlands., Mishra M; Department of Cardiothoracic Surgery, University Medical Center Utrecht, Utrecht, The Netherlands.; Laboratory of Experimental Cardiology, Department of Cardiology, University Medical Center Utrecht, University Utrecht, Utrecht, The Netherlands., van Tuijl S; LifeTec Group B.V., Kennedyplein 10-11, The Netherlands., de Jager SC; Laboratory of Experimental Cardiology, Department of Cardiology, University Medical Center Utrecht, University Utrecht, Utrecht, The Netherlands., Sluijter JP; Laboratory of Experimental Cardiology, Department of Cardiology, University Medical Center Utrecht, University Utrecht, Utrecht, The Netherlands., Doevendans PA; Department of Cardiology, University Medical Center Utrecht, University Utrecht, Utrecht, The Netherlands.; Netherlands Heart Institute, Moreelsepark 1, The Netherlands., van der Kaaij NP; Department of Cardiothoracic Surgery, University Medical Center Utrecht, Utrecht, The Netherlands.
Jazyk:	angličtina
Zdroj:	Perfusion [Perfusion] 2024 Apr; Vol. 39 (3), pp. 555-563. Date of Electronic Publication: 2023 Jan 13.
DOI:	10.1177/02676591231152718
Abstrakt:	Introduction: To validate slaughterhouse hearts for ex-situ heart perfusion studies, we compared cold oxygenated machine perfusion in less expensive porcine slaughterhouse hearts ( N = 7) to porcine hearts that are harvested following the golden standard in laboratory animals ( N = 6). Methods: All hearts received modified St Thomas 2 crystalloid cardioplegia prior to 4 hours of cold oxygenated machine perfusion. Hearts were perfused with homemade modified Steen heart solution with a perfusion pressure of 20-25 mmHg to achieve a coronary flow between 100-200 mL/min. Reperfusion and testing was performed for 4 hours on a normothermic, oxygenated diluted whole blood loaded heart model. Survival was defined by a cardiac output above 3 L with a mean aortic pressure above 60 mmHg. Results: Both groups showed 100% functional survival, with laboratory hearts displaying superior cardiac function. Both groups showed similar decline in function over time. Conclusion: We conclude that the slaughterhouse heart can be used as an alternative to laboratory hearts and provides a cost-effective method for future ex-situ heart perfusion studies. Competing Interests: Declaration of conflictintg interestsThe author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.
Databáze:	MEDLINE
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