| Autor: |
Gomes JT; Department of Dentistry, Federal University of Pará, Guamá, Belém, Pará 66075-970, Brazil., Wanzeler AMV; Department of Dentistry, Federal University of Pará, Guamá, Belém, Pará 66075-970, Brazil., Júnior SMA; Department of Dentistry, Federal University of Pará, Guamá, Belém, Pará 66075-970, Brazil., Soares RHFC; Department of Medicine, University Center of Pará, Souza, Belém, Pará 66613-903, Brazil., Oliveira CP; Department of Medicine, University Center of Pará, Souza, Belém, Pará 66613-903, Brazil., Rodrigues EM; Department of Medicine, University Center of Pará, Souza, Belém, Pará 66613-903, Brazil., Soares BM; Department of Genetics, Laboratory of Human Cytogenetics, Federal University of Pará, Guamá, Belém, Pará 66075-970, Brazil., Alcantara DDFA; Department of Genetics, Laboratory of Human Cytogenetics, Federal University of Pará, Guamá, Belém, Pará 66075-970, Brazil., Burbano RMR; Department of Genetics, Laboratory of Human Cytogenetics, Federal University of Pará, Guamá, Belém, Pará 66075-970, Brazil., Tuji FM; Department of Dentistry, Federal University of Pará, Guamá, Belém, Pará 66075-970, Brazil. |
| Abstrakt: |
The oral mucositis is a mucosal alteration that usually arises from oncological treatments, such as chemotherapy, and it is characterized as an inflammatory process. The aim of this study is to demonstrate the chromatographic constitution of Andiroba oil, comparing and evaluating Andiroba oil and laser scarring efficiency in treatments of oral mucositis in hamsters. These animals were submitted to 5-Fluorouracil. A total of 122 animals were used, randomized and divided into the following groups: (a) positive control; (b) laser associated to andiroba oil; (c) laser; (d) andiroba oil; (e) negative control; (f) cyclophosphamide (genotoxicity control). The induction of oral mucositis occurred by the administration of intraperitoneal Fluorouracila (60 mg/kg) and trauma to the mucosa. The laser protocol was performed once a day and the andiroba oil applied 3 times a day (1,5 ml/day). The mucosae were photographed and removed for clinical and histopathological analysis on day 4, 8, 12 and 15. The analysis was based in OM severity, in specific scoring for the clinical and histopathological aspect. Toxicity was evaluated on day 15 using comet assay and it was performed by variant DNA damage parameters. The data were analysed using analysis of variance (ANOVA) Tukey post -test and Kruskal-Wallis Dunn post -test . The "andiroba oil" and "laser" groups presented better results when compared to the control groups and the treatment associations. The andiroba oil presented the best scarring results, even considering its efficiency proximity to the laser treatment. Andiroba and laser, separately, did not present genotoxicity, however their association evidences damage to DNA. |
