| Autor: |
Gryffroy L; Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium.; VIB Center for Plant Systems Biology, 9052 Ghent, Belgium., Ceulemans E; Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium.; VIB Center for Plant Systems Biology, 9052 Ghent, Belgium., Manosalva Pérez N; Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium.; VIB Center for Plant Systems Biology, 9052 Ghent, Belgium., Venegas-Molina J; Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium.; VIB Center for Plant Systems Biology, 9052 Ghent, Belgium., Jaramillo-Madrid AC; Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium.; VIB Center for Plant Systems Biology, 9052 Ghent, Belgium., Rodrigues SD; Department of Biosystems, Division of Crop Biotechnics, Katholieke Universiteit Leuven, 3001 Leuven, Belgium., De Milde L; Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium.; VIB Center for Plant Systems Biology, 9052 Ghent, Belgium., Jonckheere V; Department of Biosystems, Division of Crop Biotechnics, Katholieke Universiteit Leuven, 3001 Leuven, Belgium., Van Montagu M; Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium.; VIB Center for Plant Systems Biology, 9052 Ghent, Belgium., De Coninck B; Department of Biosystems, Division of Crop Biotechnics, Katholieke Universiteit Leuven, 3001 Leuven, Belgium., Vandepoele K; Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium.; VIB Center for Plant Systems Biology, 9052 Ghent, Belgium., Van Damme P; Laboratory of Microbiology, Department of Biochemistry and Microbiology, Ghent University, 9000 Ghent, Belgium., Goossens A; Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium.; VIB Center for Plant Systems Biology, 9052 Ghent, Belgium. |
| Abstrakt: |
Rhizogenic Agrobacterium strains comprise biotrophic pathogens that cause hairy root disease (HRD) on hydroponically grown Solanaceae and Cucurbitaceae crops, besides being widely explored agents for the creation of hairy root cultures for the sustainable production of plant-specialized metabolites. Hairy root formation is mediated through the expression of genes encoded on the T-DNA of the root-inducing (Ri) plasmid, of which several, including root oncogenic locus B ( rolB ), play a major role in hairy root development. Despite decades of research, the exact molecular function of the proteins encoded by the rol genes remains enigmatic. Here, by means of TurboID-mediated proximity labeling in tomato ( Solanum lycopersicum) hairy roots, we identified the repressor proteins TOPLESS (TPL) and Novel Interactor of JAZ (NINJA) as direct interactors of RolB. Although these interactions allow RolB to act as a transcriptional repressor, our data hint at another in planta function of the RolB oncoprotein. Hence, by a series of plant bioassays, transcriptomic and DNA-binding site enrichment analyses, we conclude that RolB can mitigate the TPL functioning so that it leads to a specific and partial reprogramming of phytohormone signaling, immunity, growth, and developmental processes. Our data support a model in which RolB manipulates host transcription, at least in part, through interaction with TPL, to facilitate hairy root development. Thereby, we provide important mechanistic insights into this renowned oncoprotein in HRD. |
