Moxifloxacin as a contrast agent of two-photon microscopic imaging for detecting colorectal diseases.

Autor: Chen Y; Digestive Endoscopy Department, Jiangsu Province Hospital and The First Affiliated Hospital with Nanjing Medical University, Nanjing, China.; Department of General Surgery, Jiangsu Province Hospital and The First Affiliated Hospital with Nanjing Medical University, Nanjing, China.; Department of Hematology, Lymphoma Research Center, Peking University Third Hospital, Beijing, China., Xu X; National Laboratory of Solid State Microstructure of Nanjing University, Nanjing, China., Wang M; Digestive Endoscopy Department, Jiangsu Province Hospital and The First Affiliated Hospital with Nanjing Medical University, Nanjing, China.; Department of General Surgery, Jiangsu Province Hospital and The First Affiliated Hospital with Nanjing Medical University, Nanjing, China., Wang X; Digestive Endoscopy Department, Jiangsu Province Hospital and The First Affiliated Hospital with Nanjing Medical University, Nanjing, China.; Department of General Surgery, Jiangsu Province Hospital and The First Affiliated Hospital with Nanjing Medical University, Nanjing, China., Wang Y; Digestive Endoscopy Department, Jiangsu Province Hospital and The First Affiliated Hospital with Nanjing Medical University, Nanjing, China.; Department of General Surgery, Jiangsu Province Hospital and The First Affiliated Hospital with Nanjing Medical University, Nanjing, China., Zhang Y; National Laboratory of Solid State Microstructure of Nanjing University, Nanjing, China., Zhao L; Digestive Endoscopy Department, Jiangsu Province Hospital and The First Affiliated Hospital with Nanjing Medical University, Nanjing, China.; Department of General Surgery, Jiangsu Province Hospital and The First Affiliated Hospital with Nanjing Medical University, Nanjing, China., Fan Z; Digestive Endoscopy Department, Jiangsu Province Hospital and The First Affiliated Hospital with Nanjing Medical University, Nanjing, China.; Department of General Surgery, Jiangsu Province Hospital and The First Affiliated Hospital with Nanjing Medical University, Nanjing, China., Liu L; Digestive Endoscopy Department, Jiangsu Province Hospital and The First Affiliated Hospital with Nanjing Medical University, Nanjing, China.; Department of General Surgery, Jiangsu Province Hospital and The First Affiliated Hospital with Nanjing Medical University, Nanjing, China.; Gusu College of Nanjing Medical University, Suzhou, China.
Jazyk: angličtina
Zdroj: Journal of biophotonics [J Biophotonics] 2023 May; Vol. 16 (5), pp. e202200367. Date of Electronic Publication: 2023 Jan 20.
DOI: 10.1002/jbio.202200367
Abstrakt: Since two-photon microscopy (TPM) can obtain high-resolution images at cellular and subcellular level and moxifloxacin has multiphoton fluorescence characteristic, our study aimed to explore the feasibility and diagnostic value of moxifloxacin-assisted TPM in different human colorectal diseases, including low-grade intraepithelial neoplasia (LGIN), high-grade intraepithelial neoplasia (HGIN) and cancer tissues. Excitation power for TPM imaging with and without moxifloxacin was (2.74 ± 0.16) mW and (0.28 ± 0.02) mW, respectively (p < 0.05). Whether labeled with moxifloxacin or not, images of normal, LGIN, HGIN and cancer tissues all reached the strongest signal at 30 μm from the mucosa. Normalized fluorescence intensity of TPM images with moxifloxacin was approximately 10 times stronger than that without moxifloxacin. Fluorescence signal was differed significantly in normal, LGIN, HGIN and cancer tissues with or without moxifloxacin (p < 0.05). Besides, moxifloxacin-assisted TPM could present variant tissue features with different colorectal diseases, such as the crypt opening, glandular structure, adjacent glandular space and fluorescence distribution.
(© 2023 Wiley-VCH GmbH.)
Databáze: MEDLINE
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