Tomato SlGSTU38 interacts with the PepMV coat protein and promotes viral infection.

Autor: Méndez-López E; Department of Stress Biology and Plant Pathology, CEBAS-CSIC, Campus Universitario de Espinardo, 30100, Murcia, Spain., Donaire L; Department of Stress Biology and Plant Pathology, CEBAS-CSIC, Campus Universitario de Espinardo, 30100, Murcia, Spain., Gosálvez B; Department of Stress Biology and Plant Pathology, CEBAS-CSIC, Campus Universitario de Espinardo, 30100, Murcia, Spain., Díaz-Vivancos P; Department of Plant Breeding, CEBAS-CSIC, Campus Universitario de Espinardo, 30100, Murcia, Spain., Sánchez-Pina MA; Department of Stress Biology and Plant Pathology, CEBAS-CSIC, Campus Universitario de Espinardo, 30100, Murcia, Spain., Tilsner J; Biomedical Sciences Research Complex, The University of St. Andrews, St. Andrews, KY16 9ST, UK.; Cell and Molecular Sciences, The James Hutton Institute, Dundee, DD2 5DA, UK., Aranda MA; Department of Stress Biology and Plant Pathology, CEBAS-CSIC, Campus Universitario de Espinardo, 30100, Murcia, Spain.
Jazyk: angličtina
Zdroj: The New phytologist [New Phytol] 2023 Apr; Vol. 238 (1), pp. 332-348. Date of Electronic Publication: 2023 Feb 04.
DOI: 10.1111/nph.18728
Abstrakt: Pepino mosaic virus (PepMV) is pandemic in tomato crops, causing important economic losses world-wide. No PepMV-resistant varieties have been developed yet. Identification of host factors interacting with PepMV proteins is a promising source of genetic targets to develop PepMV-resistant varieties. The interaction between the PepMV coat protein (CP) and the tomato glutathione S-transferase (GST) SlGSTU38 was identified in a yeast two-hybrid (Y2H) screening and validated by directed Y2H and co-immunoprecipitation assays. SlGSTU38-knocked-out Micro-Tom plants (gstu38) generated by the CRISPR/Cas9 technology together with live-cell imaging were used to understand the role of SlGSTU38 during infection. The transcriptomes of healthy and PepMV-infected wild-type (WT) and gstu38 plants were profiled by RNA-seq analysis. SlGSTU38 functions as a PepMV-specific susceptibility factor in a cell-autonomous manner and relocalizes to the virus replication complexes during infection. Besides, knocking out SlGSTU38 triggers reactive oxygen species accumulation in leaves and the deregulation of stress-responsive genes. SlGSTU38 may play a dual role: On the one hand, SlGSTU38 may exert a proviral function depending on its specific interaction with the PepMV CP; and on the other hand, SlGSTU38 may delay PepMV-infection sensing by participating in the redox intracellular homeostasis in a nonspecific manner.
(© 2023 The Authors. New Phytologist © 2023 New Phytologist Foundation.)
Databáze: MEDLINE
Externí odkaz: