Enhancing the Effect of Placental Extract on the Regeneration of Crush Injured Facial Nerve.

Autor:	Lim GM; BK21 FOUR Education Research Group for Age-Associated Disorder Control Technology, Department of Integrative Biological Science, Chosun University, Gwangju 61452, Korea., Cho GW; BK21 FOUR Education Research Group for Age-Associated Disorder Control Technology, Department of Integrative Biological Science, Chosun University, Gwangju 61452, Korea.; Department of Biology, College of Natural Science, Chosun University, Gwangju 61452, Korea., Ganesan CD; Department of Biology, College of Natural Science, Chosun University, Gwangju 61452, Korea., Choi JH; Department of Obstetrics and Gynecology, Chosun University School of Medicine, Gwangju 61452, Korea., Ang MJ; Department of Veterinary Anatomy, College of Veterinary Medicine and BK21 FOUR Program, Chonnam National University, Gwangju 61186, Korea., Moon C; Department of Veterinary Anatomy, College of Veterinary Medicine and BK21 FOUR Program, Chonnam National University, Gwangju 61186, Korea., Jang CH; Department of Otolaryngology, Chonnam National University Medical School, Gwangju 61469, Korea.
Jazyk:	angličtina
Zdroj:	Experimental neurobiology [Exp Neurobiol] 2022 Dec 31; Vol. 31 (6), pp. 419-430.
DOI:	10.5607/en22006
Abstrakt:	There is a scarcity of experimental studies on peripheral nerve regeneration using placental extract (PE). This study aimed to investigate the effects of topical PE application on recovery after crush injury to the rat facial nerve using functional, electrophysiological, and morphological evaluations. The viability of the RSC96 Schwann cells treated with PE (0.5~4 mg/ml) increased significantly. Immunoblot test revealed that PE application enhanced the migration of RSC96 cells. Quantitative polymerase chain reaction demonstrated that PE increased the expression of neurotropic genes. The recovery from vibrissa fibrillation in the PE-treated group was superior to that in the control group. The threshold of action potential was also significantly lower in the PE group. Histopathological examination showed that crushed facial nerves treated with PE exhibited larger axons. The surrounding myelin sheaths were more distinct and thicker in the PE-treated group. Hence, PE may be considered a topical therapeutic agent for treating traumatic facial nerve paralysis.
Databáze:	MEDLINE
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