Differential gene expression in the contralateral hemisphere of the rat brain after focal ischemia.

Autor: Filippenkov IB; Institute of Molecular Genetics of National Research Center 'Kurchatov Institute', Kurchatov Sq. 2, 123182, Moscow, Russia. filippenkov@img.msk.ru., Remizova JA; Institute of Molecular Genetics of National Research Center 'Kurchatov Institute', Kurchatov Sq. 2, 123182, Moscow, Russia., Denisova AE; Department of Neurology, Neurosurgery and Medical Genetics, Pirogov Russian National Research Medical University, Ostrovitianov Str. 1, 117997, Moscow, Russia., Stavchansky VV; Institute of Molecular Genetics of National Research Center 'Kurchatov Institute', Kurchatov Sq. 2, 123182, Moscow, Russia., Golovina KD; Institute of Molecular Genetics of National Research Center 'Kurchatov Institute', Kurchatov Sq. 2, 123182, Moscow, Russia., Gubsky LV; Department of Neurology, Neurosurgery and Medical Genetics, Pirogov Russian National Research Medical University, Ostrovitianov Str. 1, 117997, Moscow, Russia.; Federal Center for the Brain and Neurotechnologies, Federal Biomedical Agency, Ostrovitianov Str. 1, Building 10, 117997, Moscow, Russia., Limborska SA; Institute of Molecular Genetics of National Research Center 'Kurchatov Institute', Kurchatov Sq. 2, 123182, Moscow, Russia., Dergunova LV; Institute of Molecular Genetics of National Research Center 'Kurchatov Institute', Kurchatov Sq. 2, 123182, Moscow, Russia.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2023 Jan 11; Vol. 13 (1), pp. 573. Date of Electronic Publication: 2023 Jan 11.
DOI: 10.1038/s41598-023-27663-8
Abstrakt: Ischemic stroke is one of the most severe polygenic brain diseases. Here, we performed further functional genetic analysis of the processes occurring in the contralateral hemisphere (CH) after ischemia-reperfusion injury in rat brain. Comparison of RNA sequencing data for subcortical samples from the ipsilateral hemisphere (IH) and CH after 90 min of transient middle cerebral artery occlusion (tMCAO) and corresponding sham-operated (SO) controls showed four groups of genes that were associated with ischemic processes in rat brain at 24 h after tMCAO. Among them, 2672 genes were differentially expressed genes (DEGs) for IH but non-DEGs for CH, 34 genes were DEGs for CH but non-DEGs for IH, and 114 genes had codirected changes in expression in both hemispheres. The remaining 16 genes exhibited opposite changes at the mRNA level in the two brain hemispheres after tMCAO. These findings suggest that the ischemic process caused by a focal ischemia induces complex bilateral reactions at the transcriptome level in the rat brain. We believe that specific genome responses in the CH and IH may provide a useful model for the study of the potential for brain repair after stroke.
(© 2023. The Author(s).)
Databáze: MEDLINE
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