Low levels of TNFA gene expression seem to favor the development of pulmonary tuberculosis in a population from the Brazilian Amazon.

Autor: Sousa FDM; Laboratory of Virology, Institute of Biological Sciences, Federal University of Pará (UFPA), Belém 66.075-110, Brazil; Graduate Program in Biology of Infectious and Parasitic Agents, Institute of Biological Sciences, Federal University of Pará, Belém, Pará, Brazil., Souza IP; Laboratory of Virology, Institute of Biological Sciences, Federal University of Pará (UFPA), Belém 66.075-110, Brazil; Graduate Program in Biology of Infectious and Parasitic Agents, Institute of Biological Sciences, Federal University of Pará, Belém, Pará, Brazil., Amoras EDSG; Laboratory of Virology, Institute of Biological Sciences, Federal University of Pará (UFPA), Belém 66.075-110, Brazil., Lima SS; Laboratory of Virology, Institute of Biological Sciences, Federal University of Pará (UFPA), Belém 66.075-110, Brazil., Cayres-Vallinoto IMV; Laboratory of Virology, Institute of Biological Sciences, Federal University of Pará (UFPA), Belém 66.075-110, Brazil., Ishak R; Laboratory of Virology, Institute of Biological Sciences, Federal University of Pará (UFPA), Belém 66.075-110, Brazil., Vallinoto ACR; Laboratory of Virology, Institute of Biological Sciences, Federal University of Pará (UFPA), Belém 66.075-110, Brazil., Queiroz MAF; Laboratory of Virology, Institute of Biological Sciences, Federal University of Pará (UFPA), Belém 66.075-110, Brazil. Electronic address: alicefarma@hotmail.com.
Jazyk: angličtina
Zdroj: Immunobiology [Immunobiology] 2023 Mar; Vol. 228 (2), pp. 152333. Date of Electronic Publication: 2023 Jan 06.
DOI: 10.1016/j.imbio.2023.152333
Abstrakt: TNF-α is a Th1 cytokine profile active in the control of Mycobacterium tuberculosis infection, IL-10 is associated with persistence of bacterial infection. The aim of the study was to investigate the association of TNFA -308G/A and IL10 -819C/T polymorphisms and TNFA and IL10 gene expression levels with pulmonary and extrapulmonary tuberculosis (n = 200) and control (n = 200). The individuals were submitted to genotyping and quantification of gene expression performed by real-time quantitative polymerase chain reaction (qPCR). No association was observed between the frequencies of polymorphisms evaluated and pulmonary tuberculosis. The frequency of polymorphic genotypes for TNFA -308G/A were associated with the extrapulmonary tuberculosis (p = 0.0445). The levels of TNFA expression were lower in the pulmonary tuberculosis group than in the control (p = 0.0009). There was a positive correlation between the levels of TNFA and IL10 in patients with pulmonary tuberculosis (r = 0.560; p = 0.0103). Reduced levels of TNFA expression may promote the formation of an anti-inflammatory microenvironment, favoring the persistence of the bacillus in the host, contributing to the establishment of pulmonary tuberculosis.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 The Authors. Published by Elsevier GmbH.. All rights reserved.)
Databáze: MEDLINE
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