Tephrosia toxicaria (Sw.) Pers. extracts: Screening by examining aedicidal action under laboratory and field conditions along with its antioxidant, antileishmanial, and antimicrobial activities.

Autor: Sá GCDS; Department of Cellular Biology and Genetics, Universidade Federal do Rio Grande do Norte, Natal, Rio Grande do Norte, Brazil.; Instituto de Medicina Tropical do Rio Grande do Norte, Natal, Rio Grande do Norte, Brazil., da Silva LB; Department of Cellular Biology and Genetics, Universidade Federal do Rio Grande do Norte, Natal, Rio Grande do Norte, Brazil.; Instituto de Medicina Tropical do Rio Grande do Norte, Natal, Rio Grande do Norte, Brazil., Bezerra PVV; Department of Cellular Biology and Genetics, Universidade Federal do Rio Grande do Norte, Natal, Rio Grande do Norte, Brazil.; Instituto de Medicina Tropical do Rio Grande do Norte, Natal, Rio Grande do Norte, Brazil., da Silva MAF; Department of Cellular Biology and Genetics, Universidade Federal do Rio Grande do Norte, Natal, Rio Grande do Norte, Brazil.; Instituto de Medicina Tropical do Rio Grande do Norte, Natal, Rio Grande do Norte, Brazil., Inacio CLS; Department of Microbiology and Parasitology, Laboratory of Entomology Research, Universidade Federal do Rio Grande do Norte, Natal, Rio Grande do Norte, Brazil., Paiva WS; Department of Biochemistry, Laboratory of Biotechnology of Natural Polymer, Universidade Federal do Rio Grande do Norte, Natal, Rio Grande do Norte, Brazil., E Silva VPM; Department of Microbiology and Parasitology, Laboratory of Entomology Research, Universidade Federal do Rio Grande do Norte, Natal, Rio Grande do Norte, Brazil., Cordeiro LV; Department of Pharmaceutical Sciences, Laboratory of Mycology, Universidade Federal da Paraiba, João Pessoa, Paraiba, Brazil., Oliveira JWF; Department of Clinical and Toxicological Analysis, Laboratory of Immunoparasitology, Universidade Federal do Rio Grande do Norte, Natal, RN, Brazil., Silva MS; Department of Clinical and Toxicological Analysis, Laboratory of Immunoparasitology, Universidade Federal do Rio Grande do Norte, Natal, RN, Brazil., Lima EO; Department of Pharmaceutical Sciences, Laboratory of Mycology, Universidade Federal da Paraiba, João Pessoa, Paraiba, Brazil., Moreira FJC; Laboratory of Seeds Plant Health, Instituto Federal de Educação, Ciência e Tecnologia do Ceará, Sobral, Ceará, Brazil., Rocha HAO; Department of Biochemistry, Laboratory of Biotechnology of Natural Polymer, Universidade Federal do Rio Grande do Norte, Natal, Rio Grande do Norte, Brazil., Barra PB; Department of Biomedical Sciences, Universidade do Estado do Rio Grande do Norte, Mossoró, Rio Grande do Norte, Brazil., Ximenes MFFM; Department of Microbiology and Parasitology, Laboratory of Entomology Research, Universidade Federal do Rio Grande do Norte, Natal, Rio Grande do Norte, Brazil., Uchôa AF; Department of Cellular Biology and Genetics, Universidade Federal do Rio Grande do Norte, Natal, Rio Grande do Norte, Brazil.; Instituto de Medicina Tropical do Rio Grande do Norte, Natal, Rio Grande do Norte, Brazil.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2023 Jan 11; Vol. 18 (1), pp. e0275835. Date of Electronic Publication: 2023 Jan 11 (Print Publication: 2023).
DOI: 10.1371/journal.pone.0275835
Abstrakt: An increase in the incidence of arboviral, microbial and parasitic infections, and to disorders related to oxidative stress has encouraged the development of adjuvant therapies based on natural formulations, such as those involving plant extracts. Thus, to expand the repertoire of the available therapeutic options, this study aimed to describe the versatility of Tephrosia toxicaria (Sw.) (Pers., 1807) extracts for the control of arbovirus vectors, as well as their antioxidant, antileishmanial, and antimicrobial potential. Among the aqueous and hydroethanolic extracts obtained, the hydroethanolic extract from roots (RHA) was identified as the most active larvicide extract demonstrating, respectively, the lowest lethal concentration (mg/mL) for 50%, 90% and 99% of Aedes aegypti (L., 1762) and Aedes albopictus (S., 1894) larvae, observed at 24 h (0.33, 0.84 and 1.80; 0.32, 0.70 and 1.32) and 48 h (0.17, 0.51 and 1.22; 0.26, 0.47 and 0.78) post-exposure. Field assays revealed that RHA (0.84 mg/mL) is a potential oviposition deterrent, reducing egg-laying by approximately 90%. RHA (0.1 mg/mL) also exhibited antioxidant activity for the following tests: total antioxidant capacity (286.86 mg AAE/g), iron (87.16%) and copper (25.64%) chelation, and superoxide scavenging (10%). In the cell culture assays, RHA (0.1 mg/mL) promoted regeneration of metabolic activity (92% cell viability) in cells exposed to oxidative stress. Furthermore, RHA displayed weak antileishmanial activity (IC50 = 3.53 mg/mL) against Leishmania amazonensis and not exhibit antimicrobial activity. The extraction favored the concentration of carbohydrates in RHA, in addition to lectins and protease inhibitors, with molecular masses estimated between 10 and 24 kDa. Cytotoxicity and phytotoxicity analyses of RHA suggested its biosecurity. Thus, RHA is a multivalent extract with insecticide and antioxidant properties at low and safe concentrations. However, others studies on its indirect toxic effects are ongoing to ensure the complete safety of RHA.
Competing Interests: The authors have declared no competing interests exist.
(Copyright: © 2023 Sá et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
Databáze: MEDLINE
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