Reduced acetic acid formation using NaHSO 3 as a steering agent by Actinobacillus succinogenes GXAS137.

Autor: Shen N; Guangxi Key Laboratory for Polysaccharide Materials and Modifications, Guangxi Key Laboratory of Microbial Plant Resources and Utilization, School of Marine Sciences and Biotechnology, Guangxi Minzu University, Nanning 530008, China., Li S; Guangxi Key Laboratory for Polysaccharide Materials and Modifications, Guangxi Key Laboratory of Microbial Plant Resources and Utilization, School of Marine Sciences and Biotechnology, Guangxi Minzu University, Nanning 530008, China., Li S; Guangxi Key Laboratory for Polysaccharide Materials and Modifications, Guangxi Key Laboratory of Microbial Plant Resources and Utilization, School of Marine Sciences and Biotechnology, Guangxi Minzu University, Nanning 530008, China., Wang Y; Guangxi Key Laboratory for Polysaccharide Materials and Modifications, Guangxi Key Laboratory of Microbial Plant Resources and Utilization, School of Marine Sciences and Biotechnology, Guangxi Minzu University, Nanning 530008, China., Zhang H; Guangxi Key Laboratory for Polysaccharide Materials and Modifications, Guangxi Key Laboratory of Microbial Plant Resources and Utilization, School of Marine Sciences and Biotechnology, Guangxi Minzu University, Nanning 530008, China. Electronic address: hongyanzhang2008@163.com., Jiang M; Guangxi Key Laboratory for Polysaccharide Materials and Modifications, Guangxi Key Laboratory of Microbial Plant Resources and Utilization, School of Marine Sciences and Biotechnology, Guangxi Minzu University, Nanning 530008, China.
Jazyk: angličtina
Zdroj: Journal of bioscience and bioengineering [J Biosci Bioeng] 2023 Mar; Vol. 135 (3), pp. 203-209. Date of Electronic Publication: 2023 Jan 08.
DOI: 10.1016/j.jbiosc.2022.12.007
Abstrakt: The high production of acetic acid (AC) as a by-product leads to difficult separation and purification of succinic acid (SA) and increases production costs in SA fermentation by Actinobacillus succinogenes. NaHSO 3 as a steering agent was used to reduce AC production. Herein, the optimum fermentation conditions were achieved by single-factor and orthogonal tests as follows: glucose 60 g/L; MgCO 3 60 g/L; NaHSO 3 0.15% (w/v); and NaHSO 3 addition time, 8 h after inoculation. After optimization, the SA and AC contents were 44.42 and 5.73 g/L. The SA improved by 100.72%, the AC decreased by 21.18% compared with the unfermented. The acetate kinase activity decreased by 14.36% and acetyl-CoA content improved by 97.55% in the group of NaHSO 3 addition compared with control check (CK). The mechanism of NaHSO 3 is formation acetaldehyde-sodium bisulfite compound and reduction the activity of acetate kinase. These findings indicated a new way of using NaHSO 3 as a steering agent to reduce AC generation and may help promote the development of SA industrial production.
(Copyright © 2022 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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