Evaluation of 1,2-diacyl-3-acetyl triacylglycerol production in Yarrowia lipolytica.

Autor: Yan Q; Department of Chemical and Biological Engineering, University of Wisconsin-Madison, Madison, WI, 53706, USA; DOE Center for Advanced Bioenergy and Bioproducts Innovation, University of Wisconsin-Madison, Madison, WI, 53706, USA., Jacobson TB; Department of Bacteriology, University of Wisconsin-Madison, Madison, WI, USA; DOE Great Lakes Bioenergy Research Center, University of Wisconsin-Madison, Madison, WI, USA; DOE Center for Bioenergy Innovation, Oak Ridge National Laboratory, Oak Ridge, TN, USA., Ye Z; Department of Chemical and Biological Engineering, University of Wisconsin-Madison, Madison, WI, 53706, USA., Cortés-Pena YR; DOE Center for Advanced Bioenergy and Bioproducts Innovation (CABBI), University of Illinois Urbana-Champaign, 1206 W. Gregory Drive, Urbana, IL, 61801, USA; Department of Civil and Environmental Engineering, University of Illinois Urbana-Champaign, 3221 Newmark Civil Engineering Laboratory, 205 N. Mathews Avenue, Urbana, IL, 61801, USA., Bhagwat SS; DOE Center for Advanced Bioenergy and Bioproducts Innovation (CABBI), University of Illinois Urbana-Champaign, 1206 W. Gregory Drive, Urbana, IL, 61801, USA; Department of Civil and Environmental Engineering, University of Illinois Urbana-Champaign, 3221 Newmark Civil Engineering Laboratory, 205 N. Mathews Avenue, Urbana, IL, 61801, USA., Hubbard S; Department of Chemical and Biological Engineering, University of Wisconsin-Madison, Madison, WI, 53706, USA; DOE Center for Advanced Bioenergy and Bioproducts Innovation, University of Wisconsin-Madison, Madison, WI, 53706, USA., Cordell WT; Department of Chemical and Biological Engineering, University of Wisconsin-Madison, Madison, WI, 53706, USA; DOE Center for Advanced Bioenergy and Bioproducts Innovation, University of Wisconsin-Madison, Madison, WI, 53706, USA., Oleniczak RE; Department of Chemical and Biological Engineering, University of Wisconsin-Madison, Madison, WI, 53706, USA; DOE Center for Advanced Bioenergy and Bioproducts Innovation, University of Wisconsin-Madison, Madison, WI, 53706, USA., Gambacorta FV; Department of Chemical and Biological Engineering, University of Wisconsin-Madison, Madison, WI, 53706, USA; DOE Great Lakes Bioenergy Research Center, University of Wisconsin-Madison, Madison, WI, USA., Vazquez JR; Department of Bacteriology, University of Wisconsin-Madison, Madison, WI, USA; DOE Great Lakes Bioenergy Research Center, University of Wisconsin-Madison, Madison, WI, USA; DOE Center for Bioenergy Innovation, Oak Ridge National Laboratory, Oak Ridge, TN, USA; Microbiology Doctoral Training Program, University of Wisconsin-Madison, Madison, WI, 53706, USA., Shusta EV; Department of Chemical and Biological Engineering, University of Wisconsin-Madison, Madison, WI, 53706, USA; Department of Neurological Surgery, University of Wisconsin-Madison, 1415 Engineering Drive, Madison, WI, 53706, USA., Amador-Noguez D; Department of Bacteriology, University of Wisconsin-Madison, Madison, WI, USA; DOE Great Lakes Bioenergy Research Center, University of Wisconsin-Madison, Madison, WI, USA; DOE Center for Bioenergy Innovation, Oak Ridge National Laboratory, Oak Ridge, TN, USA; DOE Center for Advanced Bioenergy and Bioproducts Innovation (CABBI), University of Illinois Urbana-Champaign, 1206 W. Gregory Drive, Urbana, IL, 61801, USA., Guest JS; DOE Center for Advanced Bioenergy and Bioproducts Innovation (CABBI), University of Illinois Urbana-Champaign, 1206 W. Gregory Drive, Urbana, IL, 61801, USA; Department of Civil and Environmental Engineering, University of Illinois Urbana-Champaign, 3221 Newmark Civil Engineering Laboratory, 205 N. Mathews Avenue, Urbana, IL, 61801, USA., Pfleger BF; Department of Chemical and Biological Engineering, University of Wisconsin-Madison, Madison, WI, 53706, USA; DOE Center for Advanced Bioenergy and Bioproducts Innovation, University of Wisconsin-Madison, Madison, WI, 53706, USA; DOE Great Lakes Bioenergy Research Center, University of Wisconsin-Madison, Madison, WI, USA; Microbiology Doctoral Training Program, University of Wisconsin-Madison, Madison, WI, 53706, USA. Electronic address: brian.pfleger@wisc.edu.
Jazyk: angličtina
Zdroj: Metabolic engineering [Metab Eng] 2023 Mar; Vol. 76, pp. 18-28. Date of Electronic Publication: 2023 Jan 07.
DOI: 10.1016/j.ymben.2023.01.003
Abstrakt: Plants produce many high-value oleochemical molecules. While oil-crop agriculture is performed at industrial scales, suitable land is not available to meet global oleochemical demand. Worse, establishing new oil-crop farms often comes with the environmental cost of tropical deforestation. The field of metabolic engineering offers tools to transplant oleochemical metabolism into tractable hosts while simultaneously providing access to molecules produced by non-agricultural plants. Here, we evaluate strategies for rewiring metabolism in the oleaginous yeast Yarrowia lipolytica to synthesize a foreign lipid, 3-acetyl-1,2-diacyl-sn-glycerol (acTAG). Oils made up of acTAG have a reduced viscosity and melting point relative to traditional triacylglycerol oils making them attractive as low-grade diesels, lubricants, and emulsifiers. This manuscript describes a metabolic engineering study that established acTAG production at g/L scale, exploration of the impact of lipid bodies on acTAG titer, and a techno-economic analysis that establishes the performance benchmarks required for microbial acTAG production to be economically feasible.
Competing Interests: Declaration of competing interest Authors declare that they have no competing interests.
(Copyright © 2023 International Metabolic Engineering Society. Published by Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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