Efficacy of chemical disinfectants against SARS-CoV-2 on high-touch surface materials.

Autor: Hardison RL; Battelle Memorial Institute, Columbus, OH 43201, USA., Nelson SW; Battelle Memorial Institute, Columbus, OH 43201, USA., Limmer R; Battelle Eastern Science & Technology Center, Aberdeen, MD 21001, USA., Marx J; Battelle Eastern Science & Technology Center, Aberdeen, MD 21001, USA., Taylor BM; Battelle Eastern Science & Technology Center, Aberdeen, MD 21001, USA., James RR; Battelle Memorial Institute, Columbus, OH 43201, USA., Stewart MJ; U.S. EPA, Durham, NC 27711, USA., Lee SDD; U.S. EPA, Durham, NC 27711, USA., Calfee MW; U.S. EPA, Durham, NC 27711, USA., Ryan SP; U.S. EPA, Durham, NC 27711, USA., Howard MW; Battelle Memorial Institute, Columbus, OH 43201, USA.
Jazyk: angličtina
Zdroj: Journal of applied microbiology [J Appl Microbiol] 2022 Dec 15. Date of Electronic Publication: 2022 Dec 15.
DOI: 10.1093/jambio/lxac020
Abstrakt: Aims: This study aimed to provide operationally relevant severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) surface disinfection efficacy information.
Methods and Results: Three EPA-registered disinfectants (Vital Oxide, Peroxide, and Clorox Total 360) and one antimicrobial formulation (CDC bleach) were evaluated against SARS-CoV-2 on material coupons and were tested using Spray (no touch with contact time) and Spray & Wipe (wipe immediately post-application) methods immediately and 2 h post-contamination. Efficacy was evaluated for infectious virus, with a subset tested for viral RNA (vRNA) recovery. Efficacy varied by method, disinfectant, and material. CDC bleach solution showed low efficacy against SARS-CoV-2 (log reduction < 1.7), unless applied via Spray & Wipe. Additionally, mechanical wiping increased the efficacy of treatments against SARS-CoV-2. The recovery of vRNA post-disinfection suggested that vRNA may overestimate infectious virus remaining.
Conclusions: Efficacy depends on surface material, chemical, and disinfection procedure, and suggests that mechanical wiping alone has some efficacy at removing SARS-CoV-2 from surfaces. We observed that disinfectant treatment biased the recovery of vRNA over infectious virus.
Significance and Impact of Study: These data are useful for developing effective, real-world disinfection procedures, and inform public health experts on the utility of PCR-based surveillance approaches.
(Published by Oxford University Press on behalf of Applied Microbiology International 2022.)
Databáze: MEDLINE
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