Clioquinol and 8-hydroxyquinoline-5-sulfonamide derivatives damage the cell wall of Pythium insidiosum.

Autor: Pippi B; Graduation Program in Pharmacology, Federal University of Santa Maria, Santa Maria 97105-900, RS, Brazil., Zanette RA; Graduation Program in Biological Sciences: Pharmacology and Therapeutics, Federal University of Rio Grande do Sul, Porto Alegre 90010-150, RS, Brazil., Joaquim AR; Graduation Program in Pharmaceutical Sciences, Federal University of Rio Grande do Sul, Porto Alegre, 90010-150, RS, Brazil., Krummenauer ME; Biotechnology Center, Federal University of Rio Grande do Sul, Porto Alegre 90010-150, RS, Brazil., Merkel S; Graduation Program in Biological Sciences: Pharmacology and Therapeutics, Federal University of Rio Grande do Sul, Porto Alegre 90010-150, RS, Brazil., Reginatto P; Graduation Program in Pharmaceutical Sciences, Federal University of Rio Grande do Sul, Porto Alegre, 90010-150, RS, Brazil., Vainstein MH; Biotechnology Center, Federal University of Rio Grande do Sul, Porto Alegre 90010-150, RS, Brazil., Andrade SF; Graduation Program in Pharmaceutical Sciences, Federal University of Rio Grande do Sul, Porto Alegre, 90010-150, RS, Brazil.; Graduation Program in Agricultural and Environmental Microbiology, Federal University of Rio Grande do Sul, Porto Alegre 90010-150, RS, Brazil., Fuentefria AM; Graduation Program in Pharmaceutical Sciences, Federal University of Rio Grande do Sul, Porto Alegre, 90010-150, RS, Brazil.; Graduation Program in Agricultural and Environmental Microbiology, Federal University of Rio Grande do Sul, Porto Alegre 90010-150, RS, Brazil., Tondolo JSM; Graduation Program in Pharmacology, Federal University of Santa Maria, Santa Maria 97105-900, RS, Brazil., Loreto ÉS; Graduation Program in Pharmacology, Federal University of Santa Maria, Santa Maria 97105-900, RS, Brazil., Santurio JM; Graduation Program in Pharmacology, Federal University of Santa Maria, Santa Maria 97105-900, RS, Brazil.
Jazyk: angličtina
Zdroj: Journal of applied microbiology [J Appl Microbiol] 2022 Dec 14. Date of Electronic Publication: 2022 Dec 14.
DOI: 10.1093/jambio/lxac038
Abstrakt: Aims: To evaluate the antimicrobial activity and to determine the pharmacodynamic characteristics of three 8-hydroxyquinoline derivatives (8-HQs) against Pythium insidiosum, the causative agent of pythiosis.
Methods and Results: Antimicrobial activity was tested by broth microdilution and MTT assays. The antimicrobial mode of action was investigated using sorbitol protection assay, ergosterol binding assay, and scanning electron microscopy. Clioquinol, PH151, and PH153 were active against all isolates, with MIC values ranging from 0.25 to 2 µg ml-1. They also showed a time- and dose-dependent antimicrobial effect, damaging the P. insidiosum cell wall.
Conclusions: Together, these results reinforce the potential of 8-HQs for developing new drugs to treat pythiosis.
(© The Author(s) 2022. Published by Oxford University Press on behalf of Applied Microbiology International.)
Databáze: MEDLINE
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