High body energy reserve influences extracellular vesicles miRNA contents within the ovarian follicle.

Autor: Bastos NM; Department of Veterinary Medicine, College of Animal Science and Food Engineering, University of São Paulo, Pirassununga, São Paulo, Brazil., Goulart RS; Department of Animal Science, College of Animal Science and Food Engineering, University of São Paulo, Pirassununga, São Paulo, Brazil., Bambil DB; Department of Animal Science, College of Animal Science and Food Engineering, University of São Paulo, Pirassununga, São Paulo, Brazil., Bridi A; Department of Veterinary Medicine, College of Animal Science and Food Engineering, University of São Paulo, Pirassununga, São Paulo, Brazil., Mazzarella R; Department of Veterinary Medicine, College of Animal Science and Food Engineering, University of São Paulo, Pirassununga, São Paulo, Brazil., Alves L; Department of Veterinary Medicine, College of Animal Science and Food Engineering, University of São Paulo, Pirassununga, São Paulo, Brazil., da Silva Rosa PM; Department of Veterinary Medicine, College of Animal Science and Food Engineering, University of São Paulo, Pirassununga, São Paulo, Brazil., Neto AL; Department of Animal Reproduction, College of Veterinary Medicine and Animal Science, University of São Paulo, Pirassununga, São Paulo, Brazil., Silva SL; Department of Animal Science, College of Animal Science and Food Engineering, University of São Paulo, Pirassununga, São Paulo, Brazil., de Almeida Santana MH; Department of Animal Science, College of Animal Science and Food Engineering, University of São Paulo, Pirassununga, São Paulo, Brazil., Negrão JA; Department of Basic Science, College of Animal Science and Food Engineering, University of São Paulo, Pirassununga, São Paulo, Brazil., Pugliesi G; Department of Animal Reproduction, College of Veterinary Medicine and Animal Science, University of São Paulo, Pirassununga, São Paulo, Brazil., Meirelles FV; Department of Veterinary Medicine, College of Animal Science and Food Engineering, University of São Paulo, Pirassununga, São Paulo, Brazil., Perecin F; Department of Veterinary Medicine, College of Animal Science and Food Engineering, University of São Paulo, Pirassununga, São Paulo, Brazil., da Silveira JC; Department of Veterinary Medicine, College of Animal Science and Food Engineering, University of São Paulo, Pirassununga, São Paulo, Brazil.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2023 Jan 10; Vol. 18 (1), pp. e0280195. Date of Electronic Publication: 2023 Jan 10 (Print Publication: 2023).
DOI: 10.1371/journal.pone.0280195
Abstrakt: Aiming to evaluate the effects of increased body energy reserve (BER) in Nellore cows' reproductive efficiency, cows were fed with different nutritional plans to obtain animals with high BER (HBER; Ad libitum diet) and moderate BER (MBER: cows fed 70% of HBER group ingestion). To evaluate the BER, cows were weekly weighted and evaluated for subcutaneous fat thickness and insulin serum concentration along the experimental period. At the end of the experimental period, animals were submitted to estrous synchronization and artificial insemination. Animals were slaughtered approximately 120 h after ovulation induction and the reproductive tracts were collected for embryo recovery and samples collection. Cumulus-oocyte-complexes (COC) and follicular fluid were collected from 3-6 mm in diameter ovarian follicles to perform miRNA analysis of cumulus cells (CC) and extracellular vesicles from follicular fluid (EV FF). As expected, differences were observed among MBER and HBER groups for body weight, fat thickness, and insulin serum concentration. HBER animals showed lower ovulation and embryo recovery rates compared to MBER animals. Different miRNAs were found among CC and EV FF within groups, suggesting that the BER may influence follicular communication. This suggests that small follicles (3-6 mm diameter) are already under BER effects, which may be greater on later stages of follicular development.
Competing Interests: The authors have declared that no competing interests exist.
(Copyright: © 2023 Bastos et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
Databáze: MEDLINE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje