Degradation rates influence the ability of composite samples to represent 24-hourly means of SARS-CoV-2 and other microbiological target measures in wastewater.

Autor: Babler KM; Department of Chemical, Environmental, and Materials Engineering, University of Miami, Coral Gables, FL 33146, USA., Sharkey ME; Department of Medicine, University of Miami Miller School of Medicine, Miami, FL 33136, USA., Abelson S; Department of Public Health Sciences, Miller School of Medicine, University of Miami, Miami, FL 33136, USA., Amirali A; Department of Chemical, Environmental, and Materials Engineering, University of Miami, Coral Gables, FL 33146, USA., Benitez A; Miami-Dade Water and Sewer Department, Miami, FL 33149, USA., Cosculluela GA; Department of Chemical, Environmental, and Materials Engineering, University of Miami, Coral Gables, FL 33146, USA., Grills GS; Sylvester Comprehensive Cancer Center, University of Miami Miller School of Medicine, Miami, FL 33136, USA., Kumar N; Department of Public Health Sciences, Miller School of Medicine, University of Miami, Miami, FL 33136, USA., Laine J; Environmental Health and Safety, University of Miami, Miami, FL 33136, USA., Lamar W; Division of Occupational Health, Safety & Compliance, University of Miami Health System, Miami, FL 33136, USA., Lamm ED; Department of Chemical, Environmental, and Materials Engineering, University of Miami, Coral Gables, FL 33146, USA., Lyu J; Department of Public Health Sciences, Miller School of Medicine, University of Miami, Miami, FL 33136, USA., Mason CE; Department of Physiology and Biophysics, Weill Cornell Medical College, New York City, NY 10021, USA; The HRH Prince Alwaleed Bin Talal Bin Abdulaziz Alsaud Institute for Computational Biomedicine, Weill Cornell Medicine, New York, NY 10021, USA; The WorldQuant Initiative for Quantitative Prediction, Weill Cornell Medicine, New York, NY 10021, USA., McCabe PM; Department of Psychology, University of Miami, Coral Gables, FL 33146, USA; Department of Biomedical Engineering, University of Miami, Coral Gables, FL 33146, USA., Raghavender J; Miami-Dade Water and Sewer Department, Miami, FL 33149, USA., Reding BD; Environmental Health and Safety, University of Miami, Miami, FL 33136, USA., Roca MA; Department of Chemical, Environmental, and Materials Engineering, University of Miami, Coral Gables, FL 33146, USA., Schürer SC; Sylvester Comprehensive Cancer Center, University of Miami Miller School of Medicine, Miami, FL 33136, USA; Department of Molecular & Cellular Pharmacology, University of Miami Miller School of Medicine, Miami, FL 33136, USA; Institute for Data Science & Computing, University of Miami, Coral Gables, FL, USA., Stevenson M; Department of Medicine, University of Miami Miller School of Medicine, Miami, FL 33136, USA., Szeto A; Department of Psychology, University of Miami, Coral Gables, FL 33146, USA., Tallon JJ Jr; Facilities and Operations, University of Miami, Coral Gables, FL 33146, USA., Vidović D; Department of Molecular & Cellular Pharmacology, University of Miami Miller School of Medicine, Miami, FL 33136, USA., Zarnegarnia Y; Department of Public Health Sciences, Miller School of Medicine, University of Miami, Miami, FL 33136, USA., Solo-Gabriele HM; Department of Chemical, Environmental, and Materials Engineering, University of Miami, Coral Gables, FL 33146, USA. Electronic address: hmsolo@miami.edu.
Jazyk: angličtina
Zdroj: The Science of the total environment [Sci Total Environ] 2023 Apr 01; Vol. 867, pp. 161423. Date of Electronic Publication: 2023 Jan 06.
DOI: 10.1016/j.scitotenv.2023.161423
Abstrakt: The utility of using severe-acute respiratory syndrome coronavirus-2 (SARS-CoV-2) RNA for assessing the prevalence of COVID-19 within communities begins with the design of the sample collection program. The objective of this study was to assess the utility of 24-hour composites as representative samples for measuring multiple microbiological targets in wastewater, and whether normalization of SARS-CoV-2 by endogenous targets can be used to decrease hour to hour variability at different watershed scales. Two sets of experiments were conducted, in tandem with the same wastewater, with samples collected at the building, cluster, and community sewershed scales. The first set of experiments focused on evaluating degradation of microbiological targets: SARS-CoV-2, Simian Immunodeficiency Virus (SIV) - a surrogate spiked into the wastewater, plus human waste indicators of Pepper Mild Mottle Virus (PMMoV), Beta-2 microglobulin (B2M), and fecal coliform bacteria (FC). The second focused on the variability of these targets from samples, collected each hour on the hour. Results show that SARS-CoV-2, PMMoV, and B2M were relatively stable, with minimal degradation over 24-h. SIV, which was spiked-in prior to analysis, degraded significantly and FC increased significantly over the course of 24 h, emphasizing the possibility for decay and growth within wastewater. Hour-to-hour variability of the source wastewater was large between each hour of sampling relative to the variability of the SARS-CoV-2 levels calculated between sewershed scales; thus, differences in SARS-CoV-2 hourly variability were not statistically significant between sewershed scales. Results further provided that the quantified representativeness of 24-h composite samples (i.e., statistical equivalency compared against hourly collected grabs) was dependent upon the molecular target measured. Overall, improvements made by normalization were minimal within this study. Degradation and multiplication for other targets should be evaluated when deciding upon whether to collect composite or grab samples in future studies.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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