Implant surface physicochemistry affects keratinocyte hemidesmosome formation.

Autor: Raptopoulos M; Minnesota Dental Research Center for Biomaterials and Biomechanics, University of Minnesota, Minneapolis, Minnesota, USA.; Division of Periodontology, Department of Developmental and Surgical Sciences, University of Minnesota, Minneapolis, Minnesota, USA., Fischer NG; Minnesota Dental Research Center for Biomaterials and Biomechanics, University of Minnesota, Minneapolis, Minnesota, USA., Aparicio C; Minnesota Dental Research Center for Biomaterials and Biomechanics, University of Minnesota, Minneapolis, Minnesota, USA.; Basic and Translational Research Division, Department of Odontology, UIC Barcelona - Universitat Internacional de Catalunya, Barcelona, Spain.; IBEC - Institute for BIoengineering of Catalonia, BIST-Barcelona Institute of Science and Technology, Barcelona, Spain.
Jazyk: angličtina
Zdroj: Journal of biomedical materials research. Part A [J Biomed Mater Res A] 2023 Jul; Vol. 111 (7), pp. 1021-1030. Date of Electronic Publication: 2023 Jan 09.
DOI: 10.1002/jbm.a.37486
Abstrakt: Previous studies have shown hydrophilic/hydrophobic implant surfaces stimulate/hinder osseointegration. An analogous concept was applied here using common biological functional groups on a model surface to promote oral keratinocytes (OKs) proliferation and hemidesmosomes (HD) to extend implant lifespans through increased soft tissue attachment. However, it is unclear what physicochemistry stimulates HDs. Thus, common biological functional groups (NH 2 , OH, and CH 3 ) were functionalized on glass using silanization. Non-functionalized plasma-cleaned glass and H silanization were controls. Surface modifications were confirmed with X-ray photoelectron spectroscopy and water contact angle. The amount of bovine serum albumin (BSA) and fibrinogen, and BSA thickness, were assessed to understand how adsorbed protein properties were influenced by physicochemistry and may influence HDs. OKs proliferation was measured, and HDs were quantified with immunofluorescence for collagen XVII and integrin β4. Plasma-cleaned surfaces were the most hydrophilic group overall, while CH 3 was the most hydrophobic and OH was the most hydrophilic among functionalized groups. Modification with the OH chemical group showed the highest OKs proliferation and HD expression. The OKs response on OH surfaces appeared to not correlate to the amount or thickness of adsorbed model proteins. These results reveal relevant surface physicochemical features to favor HDs and improve implant soft tissue attachment.
(© 2023 The Authors. Journal of Biomedical Materials Research Part A published by Wiley Periodicals LLC.)
Databáze: MEDLINE
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