Detection of rabies virus via exciton energy transfer between CdTe quantum dots and Au nanoparticles.

Autor:	Liao YJ; State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi University, Nanning, Guangxi, China.; Guangxi Marine Microbial Resources Industrialization Engineering Technology Research Center, Nanning, Guangxi, China.; Key Laboratory of Protection and Utilization of Marine Resources, Guangxi University for Nationalities, Nanning, Guangxi, China., Li DD; State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi University, Nanning, Guangxi, China., Cai ZL; State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi University, Nanning, Guangxi, China., Cao Y; State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi University, Nanning, Guangxi, China., Yu DL; State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi University, Nanning, Guangxi, China., Zhang HY; State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi University, Nanning, Guangxi, China., Kiflu AB; State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi University, Nanning, Guangxi, China., Huang ZY; Guangxi Marine Microbial Resources Industrialization Engineering Technology Research Center, Nanning, Guangxi, China.; Key Laboratory of Protection and Utilization of Marine Resources, Guangxi University for Nationalities, Nanning, Guangxi, China., Li XN; State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi University, Nanning, Guangxi, China., Luo TR; State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi University, Nanning, Guangxi, China.
Jazyk:	angličtina
Zdroj:	Frontiers in veterinary science [Front Vet Sci] 2022 Dec 22; Vol. 9, pp. 1079916. Date of Electronic Publication: 2022 Dec 22 (Print Publication: 2022).
DOI:	10.3389/fvets.2022.1079916
Abstrakt:	Rabies is a fatal encephalitis caused by the rabies virus. The diagnosis of the disease depends in large part on the exposure history of the victim and clinical manifestations of the disease. Rapid rabies diagnosis is an important step in its prevention and control. Therefore, for accurate and timely diagnosis and prevention of rabies, we developed nanomaterials for a novel photoelectrochemical biosensing approach (PBA) for the rapid and reliable diagnosis of rabies virus. This approach uses high-efficiency exciton energy transfer between cadmium telluride quantum dots and Au nanoparticles and is low cost, and easy to miniaturize. By constructing PBA, rabies virus can be detected quickly and with a high degree of sensitivity and specificity; the minimum detection concentration limit for rabies virus is approximately 2.16 ffu/mL of rabies virus particles, or 2.53 × 10 1 fg/mL of rabies virus RNA. PBA could also detect rabies virus in the brain and lung tissue from rabid dogs and mice with better sensitivity than RT-PCR. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2022 Liao, Li, Cai, Cao, Yu, Zhang, Kiflu, Huang, Li and Luo.)
Databáze:	MEDLINE
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