Ingenious characterization and assessment of lentil germplasm collection to aphid Acyrthosiphon pisum stress unveils distinct responses.
| Autor: | Zafeiriou I; Institute of Plant Breeding & Genetic Resources, Hellenic Agricultural Organization - DEMETER (HAO-DEMETER), Thermi, Greece., Ntoanidou S; Institute of Plant Breeding & Genetic Resources, Hellenic Agricultural Organization - DEMETER (HAO-DEMETER), Thermi, Greece., Baira E; Benaki Phytopathological Institute, Department of Pesticides Control and Phytopharmacy, Laboratory of Pesticides' Toxicology, Athens, Kifissia, Greece., Kasiotis KM; Benaki Phytopathological Institute, Department of Pesticides Control and Phytopharmacy, Laboratory of Pesticides' Toxicology, Athens, Kifissia, Greece., Barmpouni T; Benaki Phytopathological Institute, Department of Pesticides Control and Phytopharmacy, Laboratory of Pesticides' Toxicology, Athens, Kifissia, Greece., Machera K; Benaki Phytopathological Institute, Department of Pesticides Control and Phytopharmacy, Laboratory of Pesticides' Toxicology, Athens, Kifissia, Greece., Mylona PV; Institute of Plant Breeding & Genetic Resources, Hellenic Agricultural Organization - DEMETER (HAO-DEMETER), Thermi, Greece. |
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| Jazyk: | angličtina |
| Zdroj: | Frontiers in plant science [Front Plant Sci] 2022 Dec 21; Vol. 13, pp. 1011026. Date of Electronic Publication: 2022 Dec 21 (Print Publication: 2022). |
| DOI: | 10.3389/fpls.2022.1011026 |
| Abstrakt: | Lentil cultivation is often hampered by aphid population outspreads with detrimental impacts to crop development and production, challenging food safety and agriculture sustainability. The pea aphid ( Acyrthosiphon pisum ) is a significant threat to lentil in the temperate zone rainfed systems. A set of management practices including resilient cultivars and application of insecticides have effectively controlled aphid infestation. However, the plant defense against insect pests is scantily dissected and limited to the individual components including antibiosis, antixenosis and tolerance that constitute a combination of plant stress responses. Utilizing a lentil germplasm collection, we assessed the antixenosis and aphid tolerance mechanisms in association to important morphological parameters. Physiological parameters including relative water content (RWC) measured at 24h and 48h post-aphid infestation revealed genotype-specific responses. The contents of key plant hormones including salicylic acid (SA), jasmonic acid (JA), abscisic acid (ABA) and indoleacetic acid (IAA) implicated in defense signal-transduction pathways were also determined in lentil accessions after aphid herbivory infestation. In parallel, the expression of hallmark defense genes governed by SA- and JA-signaling pathways at 24h and 48h post aphid herbivory revealed significant differentiation patterns among the accessions. An interplay of hormone crosstalk is unveiled that possibly governs defense responses and aphid resistance. Besides the metabolomic profiling of accessions under aphid herbivory indicated the indispensable role of key secondary metabolites accumulation such as flavonoids, alkaloids, phenolics and fatty acids as a front line of plant defense and a potential integration of hormone signaling pathways in metabolome reprogramming. Overall, the study presents a panorama of distinct lentil responses to aphids and a critical view of the molecular mechanisms implicated in lentil insect defense to further our insight and advance crop protection and breeding approaches in a climate changing environment. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2022 Zafeiriou, Ntoanidou, Baira, Kasiotis, Barmpouni, Machera and Mylona.) |
| Databáze: | MEDLINE |
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