| Autor: |
de Morais FAP; Technological Innovation Laboratory in the Pharmaceuticals and Cosmetics Development, State University of Maringá, Maringá 87020-900, PR, Brazil.; Department of Chemistry, State University of Maringá, Maringá 87020-900, PR, Brazil., De Oliveira ACV; Department of Chemistry, State University of Maringá, Maringá 87020-900, PR, Brazil., Balbinot RB; Technological Innovation Laboratory in the Pharmaceuticals and Cosmetics Development, State University of Maringá, Maringá 87020-900, PR, Brazil., Lazarin-Bidóia D; Technological Innovation Laboratory in the Pharmaceuticals and Cosmetics Development, State University of Maringá, Maringá 87020-900, PR, Brazil., Ueda-Nakamura T; Technological Innovation Laboratory in the Pharmaceuticals and Cosmetics Development, State University of Maringá, Maringá 87020-900, PR, Brazil., de Oliveira Silva S; Technological Innovation Laboratory in the Pharmaceuticals and Cosmetics Development, State University of Maringá, Maringá 87020-900, PR, Brazil., da Silva Souza Campanholi K; Department of Chemistry, State University of Maringá, Maringá 87020-900, PR, Brazil., da Silva Junior RC; Department of Chemistry, State University of Maringá, Maringá 87020-900, PR, Brazil., Gonçalves RS; Laboratory of Chemistry of Natural Products, Department of Chemistry, Center for Exact Sciences and Technology, Federal University of Maranhão, São Luís 65080-805, MA, Brazil., Caetano W; Department of Chemistry, State University of Maringá, Maringá 87020-900, PR, Brazil., Nakamura CV; Technological Innovation Laboratory in the Pharmaceuticals and Cosmetics Development, State University of Maringá, Maringá 87020-900, PR, Brazil. |
| Abstrakt: |
Biotin, spermine, and folic acid were covalently linked to the F127 copolymer to obtain a new drug delivery system designed for HY-loaded PDT treatment against B 16 F 10 cells. Chemical structures and binders quantification were performed by spectroscopy and spectrophotometric techniques ( 1 NMR, HABA/Avidin reagent, fluorescamine assay). Critical micelle concentration, critical micelle temperature, size, polydispersity, and zeta potential indicate the hydrophobicity of the binders can influence the physicochemical parameters. Spermine-modified micelles showed fewer changes in their physical and chemical parameters than the F127 micelles without modification. Furthermore, zeta potential measurements suggest an increase in the physical stability of these carrier systems. The phototherapeutic potential was demonstrated using hypericin-loaded formulation against B 16 F 10 cells, which shows that the combination of the binders on F127 copolymer micelles enhances the photosensitizer uptake and potentializes the photodynamic activity. |
