| Autor: |
Pitol-Palin L; Department of Diagnosis and Surgery, Araçatuba Dental School, São Paulo State University-UNESP, Araçatuba 16015-050, Brazil., Frigério PB; Department of Diagnosis and Surgery, Araçatuba Dental School, São Paulo State University-UNESP, Araçatuba 16015-050, Brazil., Moura J; Department of Diagnosis and Surgery, Araçatuba Dental School, São Paulo State University-UNESP, Araçatuba 16015-050, Brazil., Pilatti L; M3 Health Indústria e Comércio de Produtos Médicos, Odontológicos e Correlatos S.A., Jundiaí 13212-213, Brazil., de Oliveira LMJ; M3 Health Indústria e Comércio de Produtos Médicos, Odontológicos e Correlatos S.A., Jundiaí 13212-213, Brazil., Matsubara EY; M3 Health Indústria e Comércio de Produtos Médicos, Odontológicos e Correlatos S.A., Jundiaí 13212-213, Brazil., Tunchel S; M3 Health Indústria e Comércio de Produtos Médicos, Odontológicos e Correlatos S.A., Jundiaí 13212-213, Brazil., Shibli JA; M3 Health Indústria e Comércio de Produtos Médicos, Odontológicos e Correlatos S.A., Jundiaí 13212-213, Brazil.; Department of Periodontology, Dental Research Division, Guarulhos University, Guarulhos 07009-043, Brazil., Blay A; M3 Health Indústria e Comércio de Produtos Médicos, Odontológicos e Correlatos S.A., Jundiaí 13212-213, Brazil., Saska S; M3 Health Indústria e Comércio de Produtos Médicos, Odontológicos e Correlatos S.A., Jundiaí 13212-213, Brazil., Okamoto R; Department of Basic Sciences, Araçatuba Dental School, São Paulo State University-UNESP, Araçatuba 16066-840, Brazil. |
| Abstrakt: |
This study evaluated the bioactivity of 3D-printed β-tricalcium phosphate (β-TCP) scaffolds or hydroxyapatite (HA) scaffolds associated with polydioxanone (PDO) membrane (Plenum ® Guide) for guided bone regeneration in rats. Fifty-four rats were divided into three groups (n = 18 animals): autogenous bone + PDO membrane (Auto/PG); 3D-printed β-TCP + PDO membrane (TCP/PG); and 3D-printed HA + PDO membrane (HA/PG). A surgical defect in the parietal bone was made and filled with the respective scaffolds and PDO membrane. The animals were euthanized 7, 30, and 60 days after the surgical procedure for micro-CT, histomorphometric, and immunolabeling analyses. Micro-CT showed an increase in trabecular thickness and a decrease in trabecular separation, even with similar bone volume percentages between TCP/PG and HA/PG vs. Auto/PG. Histometric analysis showed increased bone formation at 30 days in the groups compared to 7 days postoperatively. Immunolabeling analysis showed an increase in proteins related to bone formation at 30 days, and both groups showed a similar immunolabeling pattern. This study concludes that 3D-printed scaffolds associated with PDO membrane (Plenum ® Guide) present similar results to autogenous bone for bone regeneration. |
