| Autor: |
Essghaier B; Department of Biology, Faculty of Sciences, University of Tunis El-Manar II, Tunis 2092, Tunisia., Dridi R; Laboratoire de Matériaux Cristallochimie et Thermodynamique Appliquée, Department of Chemistry, Faculty of Sciences of Tunis, University of Tunis El-Manar II, Tunis 2092, Tunisia., Mottola F; Department of Environmental Biological and Pharmaceutical Sciences and Technologies (DiSTABiF), University of Campania L.Vanvitelli, 81100 Caserta, Italy., Rocco L; Department of Environmental Biological and Pharmaceutical Sciences and Technologies (DiSTABiF), University of Campania L.Vanvitelli, 81100 Caserta, Italy., Zid MF; Laboratoire de Matériaux Cristallochimie et Thermodynamique Appliquée, Department of Chemistry, Faculty of Sciences of Tunis, University of Tunis El-Manar II, Tunis 2092, Tunisia., Hannachi H; Laboratory of Vegetable Productivity and Environmental Constraint LR18ES04, Department of Biology, Faculty of Sciences, University Tunis El-Manar II, Tunis 2092, Tunisia. |
| Abstrakt: |
The present paper described the first green synthesis of silver nanoparticles (AgNPs) from the extremophile plant Aeonium haworthii . The characterization of the biosynthesized silver nanoparticles was carried out by using UV-Vis, FTIR and STM analysis. The antioxidant, antidiabetic and antimicrobial properties were also reported. The newly described AgNPs were spherical in shape and had a size of 35-55 nm. The lowest IC50 values measured by the DPPH assay indicate the superior antioxidant behavior of our AgNPs as opposed to ascorbic acid. The silver nanoparticles show high antidiabetic activity determined by the inhibitory effect of α amylase as compared to the standard Acarbose. Moreover, the AgNPs inhibit bacterial growth owing to a bactericidal effect with the MIC values varying from 0.017 to 1.7 µg/mL. The antifungal action was evaluated against Candida albicans , Candida tropicalis , Candida glabrata , Candida sake and non-dermatophytic onychomycosis fungi. A strong inhibitory effect on Candida factors' virulence was observed as proteinase and phospholipase limitations. In addition, the microscopic observations show that the silver nanoparticles cause the eradication of blastospores and block filamentous morphogenesis. The combination of the antioxidant, antimicrobial and antidiabetic behaviors of the new biosynthesized silver nanoparticles highlights their promising use as natural phytomedicine agents. |
