| Autor: |
Sutormin OS; Department of Biophysics, Institute of Fundamental Biology and Biotechnology, Siberian Federal University, 660041 Krasnoyarsk, Russia., Kolosova EM; Department of Biophysics, Institute of Fundamental Biology and Biotechnology, Siberian Federal University, 660041 Krasnoyarsk, Russia., Torgashina IG; Department of Biophysics, Institute of Fundamental Biology and Biotechnology, Siberian Federal University, 660041 Krasnoyarsk, Russia., Kratasyuk VA; Department of Biophysics, Institute of Fundamental Biology and Biotechnology, Siberian Federal University, 660041 Krasnoyarsk, Russia.; Photobiology Laboratory, Institute of Biophysics, Federal Research Center 'Krasnoyarsk Science Center, Siberian Branch of the Russian Academy of Sciences', 660036 Krasnoyarsk, Russia., Kudryasheva NS; Department of Biophysics, Institute of Fundamental Biology and Biotechnology, Siberian Federal University, 660041 Krasnoyarsk, Russia.; Photobiology Laboratory, Institute of Biophysics, Federal Research Center 'Krasnoyarsk Science Center, Siberian Branch of the Russian Academy of Sciences', 660036 Krasnoyarsk, Russia., Kinstler JS; Department of Biophysics, Institute of Fundamental Biology and Biotechnology, Siberian Federal University, 660041 Krasnoyarsk, Russia., Stom DI; Department of Vertebrate Zoology and Ecology, Faculty of Biology and Soil, Irkutsk State University, 664003 Irkutsk, Russia.; Baikal Museum of the Siberian Branch of the Russian Academy of Sciences, 664520 Irkutsk, Russia.; Department of Civil and Environmental Engineering, School of Architecture, Construction and Design, Irkutsk National Research Technical University, 664074 Irkutsk, Russia. |
| Abstrakt: |
Surfactants have a widespread occurrence, not only as household detergents, but also in their application in industry and medicine. There are numerous bioassays for assessing surfactant toxicity, but investigations of their impact on biological systems at the molecular level are still needed. In this paper, luminous marine bacteria and their coupled NAD(P)H:FMN-oxidoreductase + luciferase (Red + Luc) enzyme system was applied to examine the effects of different types of surfactants, including cationic cetyltrimethylammonium bromide (CTAB), non-ionic polyoxyethylene 20 sorbitan monooleate (Tween 80) and anionic sodium lauryl sulfate (SLS), and to assess whether the Red + Luc enzyme system can be used as a more sensitive indicator of toxicity. It was shown that the greatest inhibitory effect of the surfactants on the activity of luminous bacteria and the Red + Luc enzyme system was in the presence of SLS samples. The calculated IC 50 and EC 50 values of SLS were 10 -5 M and 10 -2 M for the enzymatic and cellular assay systems, respectively. The results highlight the benefits of using the enzymatic assay system in ecotoxicology as a tool for revealing surfactant effects on intracellular proteins if the cellular membrane is damaged under a long-term exposure period in the presence of the surfactants. For this purpose, the bioluminescent enzyme-inhibition-based assay could be used as an advanced research tool for the evaluation of surfactant toxicity at the molecular level of living organisms due to its technical simplicity and rapid response time. |
