Potential Anti-Inflammatory and Chondroprotective Effect of Luzula sylvatica .

Autor: Cholet J; Unité de Nutrition Humaine, Université Clermont Auvergne, CRNH Auvergne, INRAE, F-63000 Clermont-Ferrand, France., Decombat C; Unité de Nutrition Humaine, Université Clermont Auvergne, CRNH Auvergne, INRAE, F-63000 Clermont-Ferrand, France., Delort L; Unité de Nutrition Humaine, Université Clermont Auvergne, CRNH Auvergne, INRAE, F-63000 Clermont-Ferrand, France., Gainche M; Institut de Chimie de Clermont-Ferrand, Université Clermont Auvergne, Clermont Auvergne INP, CNRS, F-63000 Clermont-Ferrand, France., Berry A; Unité de Nutrition Humaine, Université Clermont Auvergne, CRNH Auvergne, INRAE, F-63000 Clermont-Ferrand, France., Ogeron C; Institut de Chimie de Clermont-Ferrand, Université Clermont Auvergne, Clermont Auvergne INP, CNRS, F-63000 Clermont-Ferrand, France., Ripoche I; Institut de Chimie de Clermont-Ferrand, Université Clermont Auvergne, Clermont Auvergne INP, CNRS, F-63000 Clermont-Ferrand, France., Vareille-Delarbre M; Unité de Nutrition Humaine, Université Clermont Auvergne, CRNH Auvergne, INRAE, F-63000 Clermont-Ferrand, France., Vermerie M; Unité de Nutrition Humaine, Université Clermont Auvergne, CRNH Auvergne, INRAE, F-63000 Clermont-Ferrand, France., Fraisse D; Unité de Nutrition Humaine, Université Clermont Auvergne, CRNH Auvergne, INRAE, F-63000 Clermont-Ferrand, France., Felgines C; Unité de Nutrition Humaine, Université Clermont Auvergne, CRNH Auvergne, INRAE, F-63000 Clermont-Ferrand, France., Rossary A; Unité de Nutrition Humaine, Université Clermont Auvergne, CRNH Auvergne, INRAE, F-63000 Clermont-Ferrand, France., Ranouille E; Greentech, Biopôle Clermont-Limagne, 63360 Saint-Beauzire, France., Berthon JY; Greentech, Biopôle Clermont-Limagne, 63360 Saint-Beauzire, France., Tourrette A; AltoPhyto, 7 rue des Gargailles, 63370 Lempdes, France., Priam J; Dômes Pharma, 3 rue André Citroën, 63430 Pont-du-Château, France., Saunier E; Dômes Pharma, 3 rue André Citroën, 63430 Pont-du-Château, France., Troin Y; Institut de Chimie de Clermont-Ferrand, Université Clermont Auvergne, Clermont Auvergne INP, CNRS, F-63000 Clermont-Ferrand, France., Senejoux F; Unité de Nutrition Humaine, Université Clermont Auvergne, CRNH Auvergne, INRAE, F-63000 Clermont-Ferrand, France., Chalard P; Institut de Chimie de Clermont-Ferrand, Université Clermont Auvergne, Clermont Auvergne INP, CNRS, F-63000 Clermont-Ferrand, France., Caldefie-Chézet F; Unité de Nutrition Humaine, Université Clermont Auvergne, CRNH Auvergne, INRAE, F-63000 Clermont-Ferrand, France.
Jazyk: angličtina
Zdroj: International journal of molecular sciences [Int J Mol Sci] 2022 Dec 21; Vol. 24 (1). Date of Electronic Publication: 2022 Dec 21.
DOI: 10.3390/ijms24010127
Abstrakt: (1) Interest in the Juncaceae family has risen as some members have shown anti-inflammatory properties and interesting compounds. In this regard, we decided to investigate the antioxidant and anti-inflammatory properties of Luzula sylvatica , a Juncaceae not yet extensively studied, in the context of osteoarthritis. (2) The Luzula sylvatica Ethanol extract (LS-E) was used to test the production of reactive oxygen species (ROS) by leucocytes, the IL1β and PGE2 production by peripheral blood mononuclear cells (PBMCs), the production of EP4, and the activation of NFκB in THP-1, as well as the IL1β-activated normal human knee articular chondrocytes (NHAC-Kn) gene expression, grown in monolayers or maintained in alginate beads. (3) Organic acids, caffeoylquinic acids, quercetin and luteolin, compounds frequently found in this family were identified. The LS-E exhibited inhibited ROS formation. The LS-E did not affect NFκB activation and IL1β secretion but dampened the secretion of PGE2 by PBMCs and the presence of EP4 in THP-1. It also modulated the expression of NHAC-Kn in both models and inhibited the expression of several proteases and inflammatory mediators. (4) Luzula sylvatica might supply interesting antioxidant protection against cartilage damages and lessen joint inflammation, notably by decreasing PGE2 secretion in the synovial fluid. Moreover, it could act directly on chondrocytes by decreasing the expression of proteases and, thus, preventing the degradation of the extracellular matrix.
Competing Interests: The authors declare no conflict of interest.
Databáze: MEDLINE
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