New Affordable Methods for Large-Scale Isolation of Major Olive Secoiridoids and Systematic Comparative Study of Their Antiproliferative/Cytotoxic Effect on Multiple Cancer Cell Lines of Different Cancer Origins.

Autor: Papakonstantinou A; Intracellular Parasitism Laboratory, Microbiology Department, Hellenic Pasteur Institute, 11521 Athens, Greece.; Laboratory of Pharmacognosy and Natural Products Chemistry, Department of Pharmacy, National and Kapodistrian University of Athens, Panepistimiopolis Zografou, 15771 Athens, Greece., Koumarianou P; Intracellular Parasitism Laboratory, Microbiology Department, Hellenic Pasteur Institute, 11521 Athens, Greece.; Light Microscopy Unit, Hellenic Pasteur Institute, 11521 Athens, Greece., Rigakou A; Laboratory of Pharmacognosy and Natural Products Chemistry, Department of Pharmacy, National and Kapodistrian University of Athens, Panepistimiopolis Zografou, 15771 Athens, Greece., Diamantakos P; Laboratory of Pharmacognosy and Natural Products Chemistry, Department of Pharmacy, National and Kapodistrian University of Athens, Panepistimiopolis Zografou, 15771 Athens, Greece., Frakolaki E; Molecular Virology Laboratory, Microbiology Department, Hellenic Pasteur Institute, 11521 Athens, Greece., Vassilaki N; Molecular Virology Laboratory, Microbiology Department, Hellenic Pasteur Institute, 11521 Athens, Greece., Chavdoula E; Biomedical Research Division, Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology, 45110 Ioannina, Greece., Melliou E; Laboratory of Pharmacognosy and Natural Products Chemistry, Department of Pharmacy, National and Kapodistrian University of Athens, Panepistimiopolis Zografou, 15771 Athens, Greece.; World Olive Center for Health, Imittou 76, 11634 Athens, Greece., Magiatis P; Laboratory of Pharmacognosy and Natural Products Chemistry, Department of Pharmacy, National and Kapodistrian University of Athens, Panepistimiopolis Zografou, 15771 Athens, Greece., Boleti H; Intracellular Parasitism Laboratory, Microbiology Department, Hellenic Pasteur Institute, 11521 Athens, Greece.; Light Microscopy Unit, Hellenic Pasteur Institute, 11521 Athens, Greece.
Jazyk: angličtina
Zdroj: International journal of molecular sciences [Int J Mol Sci] 2022 Dec 20; Vol. 24 (1). Date of Electronic Publication: 2022 Dec 20.
DOI: 10.3390/ijms24010003
Abstrakt: Olive oil phenols (OOPs) are associated with the prevention of many human cancers. Some of these have been shown to inhibit cell proliferation and induce apoptosis. However, no systematic comparative study exists for all the investigated compounds under the same conditions, due to difficulties in their isolation or synthesis. Herein are presented innovative methods for large-scale selective extraction of six major secoiridoids from olive oil or leaves enabling their detailed investigation. The cytotoxic/antiproliferative bioactivity of these six compounds was evaluated on sixteen human cancer cell lines originating from eight different tissues. Cell viability with half-maximal effective concentrations (EC50) was evaluated after 72 h treatments. Antiproliferative and pro-apoptotic effects were also assessed for the most bioactive compounds (EC50 ≤ 50 μM). Oleocanthal (1) showed the strongest antiproliferative/cytotoxic activity in most cancer cell lines (EC50: 9−20 μM). The relative effectiveness of the six OOPs was: oleocanthal (1) > oleuropein aglycone (3a,b) > ligstroside aglycone (4a,b) > oleacein (2) > oleomissional (6a,b,c) > oleocanthalic acid (7). This is the first detailed study comparing the bioactivity of six OOPs in such a wide array of cancer cell lines, providing a reference for their relative antiproliferative/cytotoxic effect in the investigated cancers.
Databáze: MEDLINE
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