| Autor: |
Hohmann U; Department of Anatomy and Cell Biology, Martin Luther University Halle-Wittenberg, 06108 Halle (Saale), Germany., von Widdern JC; Department of Anatomy and Cell Biology, Martin Luther University Halle-Wittenberg, 06108 Halle (Saale), Germany., Ghadban C; Department of Anatomy and Cell Biology, Martin Luther University Halle-Wittenberg, 06108 Halle (Saale), Germany., Giudice MCL; Department of Cellular Biophysics, Max Planck Institute for Medical Research, 69120 Heidelberg, Germany., Lemahieu G; Department of Cellular Biophysics, Max Planck Institute for Medical Research, 69120 Heidelberg, Germany., Cavalcanti-Adam EA; Department of Cellular Biophysics, Max Planck Institute for Medical Research, 69120 Heidelberg, Germany., Dehghani F; Department of Anatomy and Cell Biology, Martin Luther University Halle-Wittenberg, 06108 Halle (Saale), Germany., Hohmann T; Department of Anatomy and Cell Biology, Martin Luther University Halle-Wittenberg, 06108 Halle (Saale), Germany. |
| Abstrakt: |
Collective behavior of cells emerges from coordination of cell-cell-interactions and is important to wound healing, embryonic and tumor development. Depending on cell density and cell-cell interactions, a transition from a migratory, fluid-like unjammed state to a more static and solid-like jammed state or vice versa can occur. Here, we analyze collective migration dynamics of astrocytes and glioblastoma cells using live cell imaging. Furthermore, atomic force microscopy, traction force microscopy and spheroid generation assays were used to study cell adhesion, traction and mechanics. Perturbations of traction and adhesion were induced via ROCK or myosin II inhibition. Whereas astrocytes resided within a non-migratory, jammed state, glioblastoma were migratory and unjammed. Furthermore, we demonstrated that a switch from an unjammed to a jammed state was induced upon alteration of the equilibrium between cell-cell-adhesion and tension from adhesion to tension dominated, via inhibition of ROCK or myosin II. Such behavior has implications for understanding the infiltration of the brain by glioblastoma cells and may help to identify new strategies to develop anti-migratory drugs and strategies for glioblastoma-treatment. |
