| Autor: |
Schmitz C; Institute of Anatomy, Faculty of Medicine, LMU Munich, 80331 Munich, Germany., Alt C; Institute of Anatomy, Faculty of Medicine, LMU Munich, 80331 Munich, Germany.; Isar Klinikum Munich, 80331 Munich, Germany.; InGeneron, Inc., Houston, TX 77054, USA., Azares AR; Molecular Cardiology Research Lab, Texas Heart Institute, Houston, TX 77030, USA., Pearce DA; Sanford Research, Sioux Falls, SD 57104, USA.; Sanford Health, Sioux Falls, SD 57117, USA.; Sanford School of Medicine, University of South Dakota, Sioux Falls, SD 57105, USA., Facile TR; Sanford Health, Sioux Falls, SD 57117, USA., Furia JP; SUN Orthopedics of Evangelical Community Hospital, Lewisburg, PA 17837, USA., Maffulli N; Department of Musculoskeletal Disorders, Faculty of Medicine and Surgery, University of Salerno, 84084 Fisciano, Italy.; Centre for Sports and Exercise Medicine, Barts and The London School of Medicine and Dentistry, Mile End Hospital, Queen Mary University of London, London E1 2AD, UK.; School of Pharmacy and Bioengineering, Guy Hilton Research Centre, Keele University School of Medicine, Stoke on Trent ST4 7QB, UK., Huang C; InGeneron, Inc., Houston, TX 77054, USA., Alt EU; Isar Klinikum Munich, 80331 Munich, Germany.; InGeneron, Inc., Houston, TX 77054, USA.; Sanford School of Medicine, University of South Dakota, Sioux Falls, SD 57105, USA.; Heart and Vascular Institute, Department of Medicine, Tulane University Health Science Center, New Orleans, LA 70112, USA. |
| Abstrakt: |
Uncultured, unmodified, autologous, adipose-derived regenerative cells (UA-ADRCs) are a safe and effective treatment option for various musculoskeletal pathologies. However, it is unknown whether the composition of the final cell suspension systematically varies with the subject's individual age, sex, body mass index and ethnicity. UA-ADRCs were isolated from lipoaspirate from n = 232 subjects undergoing elective lipoplasty using the Transpose RT system (InGeneron, Inc.; Houston, TX, USA). The UA-ADRCs were assessed for the number of nucleated cells, cell viability and the number of viable nucleated cells per gram of adipose tissue harvested. Cells from n = 37 subjects were further characterized using four-channel flow cytometry. The present study shows, for the first time, that key characteristics of UA-ADRCs can be independent of the subject's age, sex, BMI and ethnicity. This result has important implications for the general applicability of UA-ADRCs in regeneration of musculoskeletal tissue. Future studies must determine whether the independence of key characteristics of UA-ADRCs of the subject's individual age, sex, BMI and ethnicity only applies to the system used in the present study, or also to others of the more than 25 different experimental methods and commercially available systems used to isolate UA-ADRCs from lipoaspirate that have been described in the literature. |
