| Autor: |
Sousa AJO; Institute of Veterinary Medicine, Federal University of Pará (UFPA), Castanhal 66075-110, Brazil.; Federal Institute of Pará (IFPA), Castanhal 68740-970, Brazil., Gurgel HJ; Institute of Veterinary Medicine, Federal University of Pará (UFPA), Castanhal 66075-110, Brazil., Coelho PSA; Institute of Veterinary Medicine, Federal University of Pará (UFPA), Castanhal 66075-110, Brazil., Silva CRG; Institute of Veterinary Medicine, Federal University of Pará (UFPA), Castanhal 66075-110, Brazil., Araújo LHV; Institute of Veterinary Medicine, Federal University of Pará (UFPA), Castanhal 66075-110, Brazil., Nascimento HSD; Institute of Veterinary Medicine, Federal University of Pará (UFPA), Castanhal 66075-110, Brazil., Rodrigues IDSR; Institute of Veterinary Medicine, Federal University of Pará (UFPA), Castanhal 66075-110, Brazil., Pantoja LC; Institute of Veterinary Medicine, Federal University of Pará (UFPA), Castanhal 66075-110, Brazil., Cardoso TDS; Institute of Veterinary Medicine, Federal University of Pará (UFPA), Castanhal 66075-110, Brazil., Silva MD; Institute of Veterinary Medicine, Federal University of Pará (UFPA), Castanhal 66075-110, Brazil.; Federal Institute of Pará (IFPA), Castanhal 68740-970, Brazil., Torres ACC; Institute of Veterinary Medicine, Federal University of Pará (UFPA), Castanhal 66075-110, Brazil., Teixeira PPM; Institute of Veterinary Medicine, Federal University of Pará (UFPA), Castanhal 66075-110, Brazil., Miranda MDS; Institute of Veterinary Medicine, Federal University of Pará (UFPA), Castanhal 66075-110, Brazil. |
| Abstrakt: |
The technique of laparoscopic oocyte aspiration has been increasingly used in animals; however, there are few records of its use in buffaloes. To describe this technique, six suckling Murrah buffaloes aged between 3 and 5 months were used. Three laparoscopic ovum pick-ups were performed in each animal, with intervals of 15 days between surgeries, completing a total of 18 procedures. The technique used three surgical ports with optics and a high-definition video camera. The introduction of the first portal and insufflation of the abdomen was performed through the open technique, with aspiration using a 20 G needle transabdominally and a vacuum pump calibrated at 50 mmHg. The mean complete surgical time from anesthesia to the removal of the animal from the litter was 49 ± 9.8 min. There were 27.8% cases of insufflation on the wrong side of the omentum. The oocyte recovery rate of 60.3% remained within the normal range. However, the rate of viable oocytes recovered was low, with only 40.8% of those recovered undergoing in vitro embryo production (IVEP). These data demonstrate that this simple, minimally invasive technique is an excellent reproductive tool for the genetic improvement of buffalo species. |
