Association of High Immunohistochemical Expression of Minichromosome Maintenance 3 with Human Oral Squamous Cell Carcinoma-A Preliminary Study.

Autor:	Zahir R; Institute of Basic Medical Sciences, Khyber Medical University, Peshawar 25100, Pakistan., Khan ZA; Department of Oral & Maxillofacial Surgery and Diagnostic Sciences, College of Dentistry, Jouf University, Sakaka 72388, Saudi Arabia., Aleem B; Institute of Pathology & Diagnostic Medicine, Khyber Medical University, Peshawar 25100, Pakistan., Ahmad S; Department of Oral Medicine & Diagnostic Sciences, Vision College, Riyadh 0096611, Saudi Arabia., Ali A; Institute of Pathology & Diagnostic Medicine, Khyber Medical University, Peshawar 25100, Pakistan.; Institute of Cancer Sciences, University of Glasgow, Glasgow G12 8QQ, UK., Issrani R; Department of Preventive Dentistry, College of Dentistry, Jouf University, Sakaka 72388, Saudi Arabia., Alruwaili MK; Department of Preventive Dentistry, College of Dentistry, Jouf University, Sakaka 72388, Saudi Arabia.; Department of Periodontology and Endodontology, Faculty of Dental Medicine, Hokkaido University, N13W7, Kita-ku, Sapporo, Hokkaido 060-8586, Japan., Iqbal S; Department of Obstetrics & Gynecology, Vision College, Riyadh 0096611, Saudi Arabia., Alghumaiz SF; Sayyaf Dental Complex, Sakaka 72311, Saudi Arabia., Alanazi SH; The Golden Al-Miswak Dental Complex, Al Qrayyat 75911, Saudi Arabia., Umer MF; Alshifa School of Public Health, Rawalpindi 46200, Pakistan., Ullah I; Institute of Pathology & Diagnostic Medicine, Khyber Medical University, Peshawar 25100, Pakistan., Sumera; Institute of Pathology & Diagnostic Medicine, Khyber Medical University, Peshawar 25100, Pakistan., Ganji KK; Department of Preventive Dentistry, College of Dentistry, Jouf University, Sakaka 72388, Saudi Arabia.; Department of Periodontology and Implantology, Sharad Pawar Dental College & Hospital, Datta Meghe Institute of Medical Sciences, Nagpur 442001, India.
Jazyk:	angličtina
Zdroj:	Diagnostics (Basel, Switzerland) [Diagnostics (Basel)] 2022 Dec 26; Vol. 13 (1). Date of Electronic Publication: 2022 Dec 26.
DOI:	10.3390/diagnostics13010061
Abstrakt:	Background: Oral squamous cell carcinoma (OSCC) may arise from premalignant oral lesions (PMOL) in most cases. Minichromosome maintenance 3 (MCM3) is a proliferative marker that has been investigated as a potential diagnostic biomarker in the diagnosis of oral cancer. Objectives: To evaluate the association of MCM3 expression, its clinicopathologic parameters and to identify snuff (also called naswar) as a potential risk factor for changes in MCM3 expression in PMOL and OSCC. Methodology: Immunohistochemistry (IHC) of MCM3 was performed on 32 PMOL, 32 OSCC and 16 normal controls after optimization of IHC methodology. Histoscore (0-300) was used as a scoring system and seven different cut-offs were identified for analyses. Data were analyzed using various statistical tests. Results: Among the seven cutoffs, 40% strong positive cells were found to be a better cut-off as they were associated with many pathological variables (Broder's grade, Aneroth's grade, and mitotic activity). The differential MCM3 expression in oral lesions (PMOL and OSCC) was statistically significant ( p = 0.03). Moreover, MCM3 expression is raised with increased duration and frequency of snuff use. Conclusion: High MCM3 expression is associated with disease progression and is a potential indicator of malignant transformations from PMOL to OSCC. Moreover, the use of snuff is associated with MCM3 over-expression.
Databáze:	MEDLINE
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