Genotypic Diversity and Antifungal Susceptibility of Clinical Isolates of Cryptococcus Gattii Species Complex from Argentina.
Autor: | Taverna CG; Departamento Micología, Instituto Nacional de Enfermedades Infecciosas 'Dr. Carlos G. Malbrán', Ciudad Autónoma de Buenos Aires, Argentina. ctaverna@anlis.gob.ar., Arias BA; Departamento Micología, Instituto Nacional de Enfermedades Infecciosas 'Dr. Carlos G. Malbrán', Ciudad Autónoma de Buenos Aires, Argentina., Firacative C; Studies in Translational Microbiology and Emerging Diseases (MICROS) Research Group, School of Medicine and Health Sciences, Universidad de Rosario, Bogotá, Colombia., Vivot ME; Departamento Micología, Instituto Nacional de Enfermedades Infecciosas 'Dr. Carlos G. Malbrán', Ciudad Autónoma de Buenos Aires, Argentina., Szusz W; Departamento Micología, Instituto Nacional de Enfermedades Infecciosas 'Dr. Carlos G. Malbrán', Ciudad Autónoma de Buenos Aires, Argentina., Vivot W; Departamento Micología, Instituto Nacional de Enfermedades Infecciosas 'Dr. Carlos G. Malbrán', Ciudad Autónoma de Buenos Aires, Argentina., Mazza M; Departamento Micología, Instituto Nacional de Enfermedades Infecciosas 'Dr. Carlos G. Malbrán', Ciudad Autónoma de Buenos Aires, Argentina., Córdoba SB; Departamento Micología, Instituto Nacional de Enfermedades Infecciosas 'Dr. Carlos G. Malbrán', Ciudad Autónoma de Buenos Aires, Argentina., Canteros CE; Departamento Micología, Instituto Nacional de Enfermedades Infecciosas 'Dr. Carlos G. Malbrán', Ciudad Autónoma de Buenos Aires, Argentina. |
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Jazyk: | angličtina |
Zdroj: | Mycopathologia [Mycopathologia] 2023 Apr; Vol. 188 (1-2), pp. 51-61. Date of Electronic Publication: 2023 Jan 07. |
DOI: | 10.1007/s11046-022-00705-x |
Abstrakt: | The aim of this study was to determine the genotypic diversity of 22 Cryptococcus gattii species complex clinical isolates from Argentina and to place these genotypes within the diversity of clinical, veterinary and environmental isolates from Latin America. Mating type and antifungal susceptibility of the isolates were also determined. By URA5-RFLP, nine isolates were identified as molecular type VGI, 10 as VGII, one as VGIII and two as VGIV. Multilocus sequence typing (MSLT), following the International Society for Human and Animal Mycology (ISHAM) consensus MLST scheme, was used to determine the genotypic diversity. Our results suggest that, in Argentina, VGI isolates have low genetic diversity, while VGII isolates have high genetic diversity. Both isolates identified as VGIV by URA5-RFLP were genotyped by MLST as belonging to the currently named VGVI clade. From all isolates, eight sequence types (STs) were unique for Argentina, while five STs have been reported already in other countries, being of high interest the genotypes ST20 and ST7 since they belong to the subtypes VGIIa and VGIIb, respectively, which are associated with hypervirulent strains responsible for outbreaks in North America. To note, geographical analysis showed that some genotypes may be associated with some regions in Argentina. Most isolates were MATα, but we are reporting one isolate MATa for the first time in the country. Antifungal susceptibility tests showed that itraconazole, voriconazole and posaconazole had high activity against all isolates, while amphotericin B, fluconazole and 5-fluorocytosine were the least active drugs against all studied isolates. (© 2023. The Author(s), under exclusive licence to Springer Nature B.V.) |
Databáze: | MEDLINE |
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