Tuberin levels during cellular differentiation in brain development.

Autor: Fidalgo da Silva E; Department of Biomedical Sciences, University of Windsor, 401 Sunset Ave. Windsor, Ontario, N9B 3P4, Canada., Abu Khatir B; Department of Biomedical Sciences, University of Windsor, 401 Sunset Ave. Windsor, Ontario, N9B 3P4, Canada., Drouillard C; Department of Biomedical Sciences, University of Windsor, 401 Sunset Ave. Windsor, Ontario, N9B 3P4, Canada., Hinch I; Department of Biomedical Sciences, University of Windsor, 401 Sunset Ave. Windsor, Ontario, N9B 3P4, Canada., Davis GO; Department of Biomedical Sciences, University of Windsor, 401 Sunset Ave. Windsor, Ontario, N9B 3P4, Canada., Sameem M; Department of Biomedical Sciences, University of Windsor, 401 Sunset Ave. Windsor, Ontario, N9B 3P4, Canada., Patel R; Department of Biomedical Sciences, University of Windsor, 401 Sunset Ave. Windsor, Ontario, N9B 3P4, Canada., Fong J; Department of Biomedical Sciences, University of Windsor, 401 Sunset Ave. Windsor, Ontario, N9B 3P4, Canada., Lubanska D; Department of Biomedical Sciences, University of Windsor, 401 Sunset Ave. Windsor, Ontario, N9B 3P4, Canada., Porter LA; Department of Biomedical Sciences, University of Windsor, 401 Sunset Ave. Windsor, Ontario, N9B 3P4, Canada. Electronic address: lporter@uwindsor.ca.
Jazyk: angličtina
Zdroj: Differentiation; research in biological diversity [Differentiation] 2023 Mar-Apr; Vol. 130, pp. 43-50. Date of Electronic Publication: 2022 Dec 24.
DOI: 10.1016/j.diff.2022.12.004
Abstrakt: Tuberin is a member of a large protein complex, Tuberous Sclerosis Complex (TSC), and acts as a sensor for nutrient status regulating protein synthesis and cell cycle progression. Mutations in the Tuberin gene, TSC2, permits the formation of tumors that can lead to developmental defects in many organ systems, including the central nervous system. Tuberin is expressed in the brain throughout development and levels of Tuberin have been found to decrease during neuronal differentiation in cell lines in vitro. Our current work investigates the levels of Tuberin at two stages of embryonic development in vivo, and we study the mRNA and protein levels during a time course using immortalized cell lines in vitro. Our results show that total Tuberin levels are tightly regulated through developmental stages in the embryonic brain. At a cell biology level, we show that Tuberin levels are higher when cells are cultured as neurospheres, and knockdown of Tuberin results in a reduction in the number of neurospheres. This functional data supports the hypothesis that Tuberin is an important regulator of stemness and the reduction of Tuberin levels might support functional differentiation in the central nervous system. Understanding how Tuberin expression is regulated throughout neural development is essential to fully comprehend the role of this protein in several developmental and neural pathologies.
(Copyright © 2022 International Society of Differentiation. Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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