Blood concentrations of mediators released from activated neutrophils are related to the severity of alcohol-induced liver damage.

Autor: Kasztelan-Szczerbinska B; Department of Gastroenterology with Endoscopy Unit, Medical University of Lublin, Lublin, Poland., Zygo B; Department of Gastroenterology with Endoscopy Unit, Independent Public Academic Hospital No. 4 in Lublin, Lublin, Poland., Rycyk-Bojarzynska A; Department of Gastroenterology with Endoscopy Unit, Medical University of Lublin, Lublin, Poland., Surdacka A; Department of Clinical Immunology, Medical University of Lublin, Lublin, Poland., Rolinski J; Department of Clinical Immunology, Medical University of Lublin, Lublin, Poland., Cichoz-Lach H; Department of Gastroenterology with Endoscopy Unit, Medical University of Lublin, Lublin, Poland.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2023 Jan 06; Vol. 18 (1), pp. e0280068. Date of Electronic Publication: 2023 Jan 06 (Print Publication: 2023).
DOI: 10.1371/journal.pone.0280068
Abstrakt: Background: Immune dysregulation and neutrophil infiltration are hallmarks of alcohol-related liver disease (ALD). Our objective was to evaluate the blood profile of neutrophil-derived mediators [neutrophil elastase (NE), myeloperoxidase (MPO), alpha1-antitrypsin (A1AT)], and their potential relevance in ALD.
Methods: 62 patients with ALD /47 males, and 15 females, aged 49,2 ± 9,9/ were prospectively recruited and distributed according to their 1/ gender, 2/ severity of liver dysfunction (by Child-Turcotte-Pugh, MELD scores, and mDF) 3/ presence of complications of ALD complications, and followed for 90 days. 24 age- and sex-matched healthy volunteers served as the control group. Neutrophil-derived biomarkers were quantified using enzyme-linked immunosorbent assays (ELISAs).
Results: Blood concentrations of MPO and NE were significantly higher in ALD patients in comparison with controls. A1AT levels were not different. There were no gender-related differences in the studied biomarker levels. Both NE and MPO correlated with routine markers of inflammation, while NE with MELD and mDF scores. Patients with a severe ALD course i.e. MELD>20 or mDF>32, presented with significantly higher NE blood concentrations.
Conclusions: Our results point out the critical role of neutrophils in the pathogenesis of ALD. NE and MPO correlated with the intensity of inflammation, and NE was related to the severity of liver dysfunction.
Competing Interests: The authors have declared that no competing interests exist.
(Copyright: © 2023 Kasztelan-Szczerbinska et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
Databáze: MEDLINE
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