Mechanotransduction events at the physiological site of touch detection.

Autor:	Ziolkowski LH; Department of Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, United States., Gracheva EO; Department of Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, United States.; Department of Neuroscience, Yale University School of Medicine, New Haven, United States.; Program in Cellular Neuroscience, Neurodegeneration and Repair, Yale University School of Medicine, New Haven, United States.; Kavli Institute for Neuroscience, Yale University School of Medicine, New Haven, United States., Bagriantsev SN; Department of Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, United States.
Jazyk:	angličtina
Zdroj:	ELife [Elife] 2023 Jan 06; Vol. 12. Date of Electronic Publication: 2023 Jan 06.
DOI:	10.7554/eLife.84179
Abstrakt:	Afferents of peripheral mechanoreceptors innervate the skin of vertebrates, where they detect physical touch via mechanically gated ion channels (mechanotransducers). While the afferent terminal is generally understood to be the primary site of mechanotransduction, the functional properties of mechanically activated (MA) ionic current generated by mechanotransducers at this location remain obscure. Until now, direct evidence of MA current and mechanically induced action potentials in the mechanoreceptor terminal has not been obtained. Here, we report patch-clamp recordings from the afferent terminal innervating Grandry (Meissner) corpuscles in the bill skin of a tactile specialist duck. We show that mechanical stimulation evokes MA current in the afferent with fast kinetics of activation and inactivation during the dynamic phases of the mechanical stimulus. These responses trigger rapidly adapting firing in the afferent detected at the terminal and in the afferent fiber outside of the corpuscle. Our findings elucidate the initial electrogenic events of touch detection in the mechanoreceptor nerve terminal. Competing Interests: LZ, EG, SB No competing interests declared (© 2023, Ziolkowski et al.)
Databáze:	MEDLINE
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