In vivo labeling of endogenous genomic loci in C. elegans using CRISPR/dCas9.
| Autor: | Memar N; Institute for Basic Science (IBS), Ulsan 44919, South Korea., Sethi A; University College London, United Kingdom., Luehr S; Ludwig-Maximilians-University Munich, Germany., Lambie EJ; University College London, United Kingdom., Conradt B; University College London, United Kingdom. |
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| Jazyk: | angličtina |
| Zdroj: | MicroPublication biology [MicroPubl Biol] 2022 Dec 13; Vol. 2022. Date of Electronic Publication: 2022 Dec 13 (Print Publication: 2022). |
| DOI: | 10.17912/micropub.biology.000701 |
| Abstrakt: | Visualization of genomic loci with open chromatin state has been reported in mammalian tissue culture cells using a CRISPR/Cas9-based system that utilizes an EGFP-tagged endonuclease-deficient Cas9 protein (dCas9::EGFP) (Chen et al. 2013). Here, we adapted this approach for use in Caenorhabditis elegans . We generated a C. elegans strain that expresses the dCas9 protein fused to two nuclear-localized EGFP molecules (dCas9::NLS::2xEGFP::NLS) in an inducible manner. Using this strain, we report the visualization in live C. elegans embryos of two endogenous repetitive loci, rrn-4 and rrn-1 , from which 5S and 18S ribosomal RNAs are constitutively generated. (Copyright: © 2022 by the authors.) |
| Databáze: | MEDLINE |
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