Special considerations for studies of extracellular vesicles from parasitic helminths: A community-led roadmap to increase rigour and reproducibility.

Autor: White R; The University of Edinburgh, Institute of Immunology and Infection Research, School of Biological Sciences, Edinburgh, UK., Sotillo J; Instituto de Salud Carlos III, National Center for Microbiology, Majadahonda, Madrid, Spain., Ancarola ME; Department of Microbiology, School of Medicine, University of Buenos Aires, Buenos Aires, Argentina.; Institute of Research on Microbiology and Medical Parasitology (IMPaM, UBA-CONICET), University of Buenos Aires, Buenos Aires, Argentina., Borup A; Department of Clinical Medicine, Aarhus University, Aarhus, Denmark., Boysen AT; Department of Clinical Medicine, Aarhus University, Aarhus, Denmark., Brindley PJ; George Washington University, Microbiology, Immunology & Tropical Medicine, School of Medicine & Health Sciences, Washington, D.C., USA., Buzás EI; ELKH-SE Immune Proteogenomics Extracellular Vesicle Research Group, Budapest, Hungary.; HCEMM-SU Extracellular Vesicle Research Group, Budapest, Hungary., Cavallero S; Department of Public health and infectious diseases, Sapienza University of Rome, Rome, Italy., Chaiyadet S; Tropical Medicine Graduate Program, Academic Affairs, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand., Chalmers IW; Aberystwyth University, Institute of Biological, Environmental & Rural Sciences (IBERS), Aberystwyth Ceredigion, Wales, UK., Cucher MA; Department of Microbiology, School of Medicine, University of Buenos Aires, Buenos Aires, Argentina.; Institute of Research on Microbiology and Medical Parasitology (IMPaM, UBA-CONICET), University of Buenos Aires, Buenos Aires, Argentina., Dagenais M; McGill University, Institute of Parasitology, Ste-Anne-de-Bellevue, Quebec, Canada., Davis CN; Aberystwyth University, Institute of Biological, Environmental & Rural Sciences (IBERS), Aberystwyth Ceredigion, Wales, UK., Devaney E; University of Glasgow, Institute of Biodiversity, Animal Health and Comparative Medicine, College of Medical, Veterinary and Life Sciences, Glasgow, UK., Duque-Correa MA; Wellcome Sanger Institute, Parasites and Microbes, Cambridge, UK., Eichenberger RM; University of Zurich, Institute of Parasitology, Vetsuisse Faculty, Zurich, Switzerland., Fontenla S; Universidad de la República, Facultad de Medicina, Departamento de Genetica, Montevideo, Uruguay., Gasan TA; Queen's University Belfast, School of Biological Sciences, Belfast, Northern Ireland, UK., Hokke CH; Leiden University Medical Center, Parasitology, Leiden, The Netherlands., Kosanovic M; Institute for the Application of Nuclear Energy, INEP, University of Belgrade, Belgrade, Serbia., Kuipers ME; Leiden University Medical Center, Parasitology, Leiden, The Netherlands.; Utrecht University, Faculty of Veterinary Medicine, Biomolecular Health Sciences, Utrecht, The Netherlands., Laha T; Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand., Loukas A; James Cook University, Australian Institute of Tropical Health and Medicine, Cairns, Queensland, Australia., Maizels RM; University of Glasgow, Wellcome Centre for Integrative Parasitology, Institute of Infection, Immunity and Inflammation, Glasgow, UK., Marcilla A; Universitat de València, Departamento Farmacia y Tecnología Farmacéutica y Parasitología, Área de Parasitología, Burjsassot, Valencia, Spain., Mazanec H; Faculty of Science, University of South Bohemia, České Budějovice, Czech Republic.; Czech Republic, Institute of Parasitology, Biology Centre, Czech Academy of Sciences, České Budějovice, Czech Republic., Morphew RM; Aberystwyth University, Institute of Biological, Environmental & Rural Sciences (IBERS), Aberystwyth Ceredigion, Wales, UK., Neophytou K; The University of Edinburgh, Institute of Immunology and Infection Research, School of Biological Sciences, Edinburgh, UK., Nguyen LT; Department of Biochemical Sciences, Faculty of Pharmacy, Charles University, Prague, Czech Republic., Nolte-'t Hoen E; Utrecht University, Faculty of Veterinary Medicine, Biomolecular Health Sciences, Utrecht, The Netherlands., Povelones M; University of Pennsylvania School of Veterinary Medicine, Pathobiology, Philadelphia, Pennsylvania, USA., Robinson MW; Queen's University Belfast, School of Biological Sciences, Belfast, Northern Ireland, UK., Rojas A; Laboratory of Helminthology, Faculty of Microbiology, University of Costa Rica, San Pedro Montes de Oca, Costa Rica., Schabussova I; Medical University of Vienna, Institute of Specific Prophylaxis and Tropical Medicine, Vienna, Austria., Smits HH; Leiden University Medical Center, Parasitology, Leiden, The Netherlands., Sungpradit S; Department of Pre-clinic and Applied Animal Science, Faculty of Veterinary Science, Mahidol University, Nakhon Pathom, Thailand., Tritten L; University of Zurich, Institute of Parasitology, Vetsuisse Faculty, Zurich, Switzerland.; Swiss Tropical and Public Health Institute, Allschwil, Switzerland.; University of Basel, Basel, Switzerland., Whitehead B; Department of Clinical Medicine, Aarhus University, Aarhus, Denmark., Zakeri A; Department of Clinical Medicine, Aarhus University, Aarhus, Denmark., Nejsum P; Department of Clinical Medicine, Aarhus University, Aarhus, Denmark., Buck AH; The University of Edinburgh, Institute of Immunology and Infection Research, School of Biological Sciences, Edinburgh, UK., Hoffmann KF; Aberystwyth University, Institute of Biological, Environmental & Rural Sciences (IBERS), Aberystwyth Ceredigion, Wales, UK.
Jazyk: angličtina
Zdroj: Journal of extracellular vesicles [J Extracell Vesicles] 2023 Jan; Vol. 12 (1), pp. e12298.
DOI: 10.1002/jev2.12298
Abstrakt: Over the last decade, research interest in defining how extracellular vesicles (EVs) shape cross-species communication has grown rapidly. Parasitic helminths, worm species found in the phyla Nematoda and Platyhelminthes, are well-recognised manipulators of host immune function and physiology. Emerging evidence supports a role for helminth-derived EVs in these processes and highlights EVs as an important participant in cross-phylum communication. While the mammalian EV field is guided by a community-agreed framework for studying EVs derived from model organisms or cell systems [e.g., Minimal Information for Studies of Extracellular Vesicles (MISEV)], the helminth community requires a supplementary set of principles due to the additional challenges that accompany working with such divergent organisms. These challenges include, but are not limited to, generating sufficient quantities of EVs for descriptive or functional studies, defining pan-helminth EV markers, genetically modifying these organisms, and identifying rigorous methodologies for in vitro and in vivo studies. Here, we outline best practices for those investigating the biology of helminth-derived EVs to complement the MISEV guidelines. We summarise community-agreed standards for studying EVs derived from this broad set of non-model organisms, raise awareness of issues associated with helminth EVs and provide future perspectives for how progress in the field will be achieved.
(© 2023 The Authors. Journal of Extracellular Vesicles published by Wiley Periodicals, LLC on behalf of the International Society for Extracellular Vesicles.)
Databáze: MEDLINE
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