Improving cassava bacterial blight resistance by editing the epigenome.

Autor: Veley KM; Donald Danforth Plant Science Center, Saint Louis, MO, 63132, USA., Elliott K; Donald Danforth Plant Science Center, Saint Louis, MO, 63132, USA.; Division of Biological and Biomedical Sciences, Washington University in Saint Louis, Saint Louis, MO, 63110, USA., Jensen G; Donald Danforth Plant Science Center, Saint Louis, MO, 63132, USA., Zhong Z; Department of Molecular, Cell and Developmental Biology, University of California at Los Angeles, Los Angeles, CA, 90095, USA., Feng S; Department of Molecular, Cell and Developmental Biology, University of California at Los Angeles, Los Angeles, CA, 90095, USA.; College of Tropical Agriculture & Human Resources, University of Hawaii at Manoa, Hilo, HI, 96720, USA., Yoder M; Donald Danforth Plant Science Center, Saint Louis, MO, 63132, USA., Gilbert KB; Donald Danforth Plant Science Center, Saint Louis, MO, 63132, USA., Berry JC; Donald Danforth Plant Science Center, Saint Louis, MO, 63132, USA., Lin ZD; Donald Danforth Plant Science Center, Saint Louis, MO, 63132, USA., Ghoshal B; Department of Molecular, Cell and Developmental Biology, University of California at Los Angeles, Los Angeles, CA, 90095, USA.; Summerland Research and Development Centre, Agriculture and Agri-Food Canada, Summerland, BC, V0H 1Z0, Canada., Gallego-Bartolomé J; Department of Molecular, Cell and Developmental Biology, University of California at Los Angeles, Los Angeles, CA, 90095, USA.; Instituto de Biología Molecular y Celular de Plantas (IBMCP), CSIC-Universidad Politécnica de Valencia, 46011, Valencia, Spain., Norton J; College of Tropical Agriculture & Human Resources, University of Hawaii at Manoa, Hilo, HI, 96720, USA., Motomura-Wages S; College of Tropical Agriculture & Human Resources, University of Hawaii at Manoa, Hilo, HI, 96720, USA., Carrington JC; Donald Danforth Plant Science Center, Saint Louis, MO, 63132, USA., Jacobsen SE; Department of Molecular, Cell and Developmental Biology, University of California at Los Angeles, Los Angeles, CA, 90095, USA. jacobsen@ucla.edu.; Eli & Edythe Broad Center of Regenerative Medicine & Stem Cell Research, University of California at Los Angeles, Los Angeles, CA, 90095, USA. jacobsen@ucla.edu.; Howard Hughes Medical Institute, University of California at Los Angeles, Los Angeles, CA, 90095, USA. jacobsen@ucla.edu., Bart RS; Donald Danforth Plant Science Center, Saint Louis, MO, 63132, USA. rbart@danforthcenter.org.
Jazyk: angličtina
Zdroj: Nature communications [Nat Commun] 2023 Jan 05; Vol. 14 (1), pp. 85. Date of Electronic Publication: 2023 Jan 05.
DOI: 10.1038/s41467-022-35675-7
Abstrakt: Pathogens rely on expression of host susceptibility (S) genes to promote infection and disease. As DNA methylation is an epigenetic modification that affects gene expression, blocking access to S genes through targeted methylation could increase disease resistance. Xanthomonas phaseoli pv. manihotis, the causal agent of cassava bacterial blight (CBB), uses transcription activator-like20 (TAL20) to induce expression of the S gene MeSWEET10a. In this work, we direct methylation to the TAL20 effector binding element within the MeSWEET10a promoter using a synthetic zinc-finger DNA binding domain fused to a component of the RNA-directed DNA methylation pathway. We demonstrate that this methylation prevents TAL20 binding, blocks transcriptional activation of MeSWEET10a in vivo and that these plants display decreased CBB symptoms while maintaining normal growth and development. This work therefore presents an epigenome editing approach useful for crop improvement.
(© 2023. The Author(s).)
Databáze: MEDLINE
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