CdSe-Co 3 O 4 @TiO 2 nanoflower-based photoelectrochemical platform probing visible light-driven virus detection.

Autor: Ganganboina AB; International Center for Young Scientists ICYS-NAMIKI, National Institute for Materials Science, 1-1 Namiki, Tsukuba, Ibaraki, 305-0044, Japan. ganganboina.akhileshbabu@nims.go.jp., Khoris IM; Department of Bioscience, Graduate School of Science and Technology, Shizuoka University, 836 Ohya Suruga-Ku, Shizuoka, 422-8529, Japan., Konno A; Graduate School of Science and Technology, Shizuoka University, 3-5-1 Johoku, Naka-Ku, Hamamatsu, Shizuoka, 432-8561, Japan., Li TC; Department of Virology 2, National Institute of Infectious Diseases, 4-7-1 Gakuen, Musashimurayama-Shi, Tokyo, 208-0011, Japan., Okamoto A; International Center for Materials Nanoarchitectonics (WPI-MANA) and Center for Sensor and Actuator Material, National Institute for Materials Science (NIMS), 1-1 Namiki, Tsukuba, Ibaraki, 305-0044, Japan., Park EY; Department of Bioscience, Graduate School of Science and Technology, Shizuoka University, 836 Ohya Suruga-Ku, Shizuoka, 422-8529, Japan. park.enoch@shizuoka.ac.jp.; Research Institute of Green Science and Technology, Shizuoka University, 836 Ohya Suruga-Ku, Shizuoka, 422-8529, Japan. park.enoch@shizuoka.ac.jp.
Jazyk: angličtina
Zdroj: Mikrochimica acta [Mikrochim Acta] 2023 Jan 06; Vol. 190 (1), pp. 46. Date of Electronic Publication: 2023 Jan 06.
DOI: 10.1007/s00604-022-05623-9
Abstrakt: The design and construction of a visible light-driven photoelectrochemical (PEC) device is described based on a CdSe-Co 3 O 4 @TiO 2 nanoflower (NF). Moreover, an application to the ultrasensitive detection of viruses, such as hepatitis E virus (HEV), HEV-like particles (HEV-LPs), and SARS-CoV-2 spike protein in complicated lysate solution, is demonstrated. The photocurrent response output of a PEC device based on CdSe-Co 3 O 4 @TiO 2 is enhanced compared with the individual components, TiO 2 and CdSe-Co 3 O 4 . This can be attributed to the CdSe quantum dot (QD) sensitization effect and strong visible light absorption to improve overall system stability. A robust oxygen-evolving catalyst (Co 3 O 4 ) coupled at the hole-trapping site (CdSe) extends the interfacial carrier lifetime, and the energy conversion efficiency was improved. The effective hybridization between the antibody and virus resulted in a linear relationship between the change in photocurrent density and the HEV-LP concentration ranging from 10 fg mL -1 to 10 ng mL -1 , with a detection limit of 3.5 fg mL -1 . This CdSe-Co 3 O 4 @TiO 2 -based PEC device achieved considerable sensitivity, good specificity, and acceptable stability and demonstrated a significant ability to develop an upgraded device with affordable and portable biosensing capabilities.
(© 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.)
Databáze: MEDLINE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje