Visualization of Single Dynein Molecules in Mammalian Cells.
| Autor: | Tirumala NA; Centre for BioSystems Science and Engineering, Indian Institute of Science, Bengaluru, India.; Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD, USA., Ananthanarayanan V; Centre for BioSystems Science and Engineering, Indian Institute of Science, Bengaluru, India. vaish@unsw.edu.au.; EMBL Australia Node in Single Molecule Science, School of Biomedical Sciences, The University of New South Wales, Sydney, NSW, Australia. vaish@unsw.edu.au. |
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| Jazyk: | angličtina |
| Zdroj: | Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2023; Vol. 2623, pp. 87-93. |
| DOI: | 10.1007/978-1-0716-2958-1_6 |
| Abstrakt: | In vitro single-molecule imaging experiments have provided insight into the stepping behavior, force production, and activation of several molecular motors. However, due to the difficulty in visualizing single molecules of motor proteins in vivo, the physiological function and regulation of motors at the single-molecule level have not been studied widely. Here, we describe how highly inclined and laminated optical sheet (HILO) microscopy can be adapted to visualize single molecules of the motor protein cytoplasmic dynein-1 in mammalian cells with high signal-to-noise ratio and temporal resolution. (© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.) |
| Databáze: | MEDLINE |
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