Identification of vacuolar autophagic aggregates in the skeletal muscles of inbred C57BL/6NCrl mice.

Autor:	De Biase D; Department of Pharmacy, University of Salerno, Italy., Pagano TB; Department of Veterinary Medicine and Animal Production University of Naples 'Federico II', Italy., Malanga D; Department of Experimental and Clinical Medicine, University 'Magna Graecia' of Catanzaro Medical School, Italy., Russo V; Department of Veterinary Medicine and Animal Production University of Naples 'Federico II', Italy., Piegari G; Department of Veterinary Medicine and Animal Production University of Naples 'Federico II', Italy., d'Aquino I; Department of Veterinary Medicine and Animal Production University of Naples 'Federico II', Italy., Iovane V; Department of Agricultural Sciences, University of Naples 'Federico II', Italy., Scarfò M; BIOGEM-Istituto di Ricerche Genetiche, Italy., Papparella S; Department of Veterinary Medicine and Animal Production University of Naples 'Federico II', Italy., Wojcik S; Department of Anatomy and Neurobiology, Medical University of Gdansk, Poland., Paciello O; Department of Veterinary Medicine and Animal Production University of Naples 'Federico II', Italy.
Jazyk:	angličtina
Zdroj:	Laboratory animals [Lab Anim] 2023 Jun; Vol. 57 (3), pp. 247-258. Date of Electronic Publication: 2023 Jan 05.
DOI:	10.1177/00236772221138942
Abstrakt:	A comprehensive pathological analysis of inbred strains is essential to define strain-specific spontaneous lesions and to understand whether a specific phenotype results from experimental intervention or reflects a naturally occurring disease. This study aimed to report and describe a novel condition affecting the skeletal muscles of an inbred C57BL/6NCrl mouse colony characterised by large sarcoplasmic vacuoles in the muscle fibres of male mice in the subsarcolemmal spaces and the intermyofibrillary network. There was no muscle weakness, loss of ambulation or cardiac/respiratory involvement. Post-mortem evaluation and histological analysis excluded the presence of pathological accumulations or lesions in other tissues and organs. Changes were seen in fibre size, with many hypotrophic and some slightly hypertrophic fibres. Histological, immunohistochemical and molecular analyses of the vacuolar content revealed dysregulation of the autophagy machinery while ruling out a morphologically similar condition marked by the accumulation of tubular aggregates.
Databáze:	MEDLINE
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