Comprehensive analysis of circulating viral DNA in maternal plasma at population-scale using low-pass whole-genome sequencing.

Autor: Eldem V; Department of Biology, Faculty of Science, Istanbul University, Istanbul 34134, Turkey. Electronic address: vahap.eldem@istanbul.edu.tr., Kuralay SC; Department of Biology, Faculty of Science, Istanbul University, Istanbul 34134, Turkey., Özdoğan G; GENOKS Genetic Disease Diagnostic Center, Ankara 06560, Turkey., Özçelik GH; GENOKS Genetic Disease Diagnostic Center, Ankara 06560, Turkey., Aydın D; GENOKS Genetic Disease Diagnostic Center, Ankara 06560, Turkey., Çakmak G; GENOKS Genetic Disease Diagnostic Center, Ankara 06560, Turkey., Gürler MÖ; GENOKS Genetic Disease Diagnostic Center, Ankara 06560, Turkey., Çay SB; Department of Biology, Faculty of Science, Istanbul University, Istanbul 34134, Turkey., Çınar YU; Department of Biology, Faculty of Science, Istanbul University, Istanbul 34134, Turkey., Dikmen F; Department of Biology, Faculty of Science, Istanbul University, Istanbul 34134, Turkey., Yusuf I; GENOKS Genetic Disease Diagnostic Center, Ankara 06560, Turkey., Obut O; Department of Biology, Faculty of Science, Istanbul University, Istanbul 34134, Turkey., Kayalar Ö; Koc University Research Center for Translational Medicine (KUTTAM), Koc University School of Medicine, Istanbul 34010, Turkey., Zararsız GE; Department of Biostatistics, Erciyes University Medical Faculty, Kayseri 38280, Turkey; Erciyes University, Drug Application and Research Center (ERFARMA), Kayseri 38280, Turkey., Edizadeh M; GENOKS Genetic Disease Diagnostic Center, Ankara 06560, Turkey., Zararsız G; Department of Biostatistics, Erciyes University Medical Faculty, Kayseri 38280, Turkey; Erciyes University, Drug Application and Research Center (ERFARMA), Kayseri 38280, Turkey., Akdeniz E; GENOKS Genetic Disease Diagnostic Center, Ankara 06560, Turkey., Özgür H; GENOKS Genetic Disease Diagnostic Center, Ankara 06560, Turkey., Tekin IM; GENOKS Genetic Disease Diagnostic Center, Ankara 06560, Turkey.
Jazyk: angličtina
Zdroj: Genomics [Genomics] 2023 Mar; Vol. 115 (2), pp. 110556. Date of Electronic Publication: 2023 Jan 01.
DOI: 10.1016/j.ygeno.2022.110556
Abstrakt: As the most readily adopted molecular screening test, low-pass WGS of maternal plasma cell-free DNA for aneuploidy detection generates a vast amount of genomic data. This large-scale method also allows for high-throughput virome screening. NIPT sequencing data, yielding 6.57 terabases of data from 187.8 billion reads, from 12,951 pregnant Turkish women was used to investigate the prevalence and abundance of viral DNA in plasma. Among the 22 virus sequences identified in 12% of participants were human papillomavirus, herpesvirus, betaherpesvirus and anellovirus. We observed a unique pattern of circulating viral DNA with a high prevalence of papillomaviruses. The prevalence of herpesviruses/anellovirus was similar among Turkish, European and Dutch populations. Hepatitis B prevalence was remarkably low in Dutch, European and Turkish populations, but higher in China. WGS data revealed that herpesvirus/anelloviruses are naturally found in European populations. This represents the first comprehensive research on the plasma virome of pregnant Turkish women.
Competing Interests: Declaration of Competing Interest The authors declare no competing interests.
(Copyright © 2022. Published by Elsevier Inc.)
Databáze: MEDLINE
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