Safety Profile of Lutein- Versus Triamcinolone Acetonide-Based Vitreous Staining.

Autor:	Lazzara F; Department of Biomedical and Biotechnological Sciences, School of Medicine, University of Catania, Catania, Italy., Conti F; Department of Biomedical and Biotechnological Sciences, School of Medicine, University of Catania, Catania, Italy., Ferrara M; Manchester Royal Eye Hospital, Manchester University Hospitals NHS Foundation Trust, Manchester, UK., Lippera M; Manchester Royal Eye Hospital, Manchester University Hospitals NHS Foundation Trust, Manchester, UK.; Department of Biomedical Sciences, Humanitas University, Pieve Emanuele, Italy., Coppola M; Department of Ophthalmology, San Gerardo Hospital, Monza, Italy., Rossi S; Multidisciplinary Department of Medical, Surgical and Dental Sciences, University of Campania Luigi Vanvitelli, Naples, Italy., Drago F; Department of Biomedical and Biotechnological Sciences, School of Medicine, University of Catania, Catania, Italy.; Center for Research in Ocular Pharmacology-CERFO, University of Catania, Catania, Italy., Bucolo C; Department of Biomedical and Biotechnological Sciences, School of Medicine, University of Catania, Catania, Italy.; Center for Research in Ocular Pharmacology-CERFO, University of Catania, Catania, Italy., Romano MR; Department of Biomedical Sciences, Humanitas University, Pieve Emanuele, Italy.; Eye Center, Humanitas Gavazzeni-Castelli, Bergamo, Italy.
Jazyk:	angličtina
Zdroj:	Translational vision science & technology [Transl Vis Sci Technol] 2023 Jan 03; Vol. 12 (1), pp. 5.
DOI:	10.1167/tvst.12.1.5
Abstrakt:	Purpose: To assess the safety profile of a new lutein-based vitreous dye (LB-VD) formulation compared with various triamcinolone acetonide (TA) formulations with and without subsequent exposure to perfluorodecalin (PFD) in vitro. Methods: Human adult retinal pigment epithelial cells (ARPE-19) were treated with the following formulations: undiluted preserved TA (TA-BA), diluted preserved TA (D-TA-BA), preservative-free TA (TA-PF), and LB-VD. First, cell tolerability was evaluated with MTT, LDH, and ATPlite assays after 1, 5, and 30 minutes of exposure to each tested formulation. Then, cells were sequentially exposed to formulations and PFD. After 24 hours of exposure to PFD, cell tolerability was evaluated through MTT and ATPlite assays. Results: Among the formulations tested, LB-VD showed the highest levels of cell viability, cell metabolism, and cell proliferation and induced the lowest release of LDH, whereas the TA-based formulations demonstrated a cytotoxic effect on ARPE-19 cells in vitro. After subsequent 24-hour exposure to PFD, a greater reduction of cell viability was noted for all the formulations; however, this reduction was not significant only for the combination LB-VD-PFD, which was the best tolerated condition. Conclusions: LB-VD showed a better safety profile compared with all TA-based formulations, even when used in combination with PFD. Translational Relevance: In surgical practice, LB-VD may be preferred to TA-based formulations for vitreous staining in the light of its more favorable safety profile.
Databáze:	MEDLINE
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