The role of cytokines and T-bet, GATA3, ROR-γt, and FOXP3 transcription factors of T cell subsets in the natural clinical progression of Type 1 Diabetes.

Autor: Ozgur BA; Department of Medical Biology and Genetics, Faculty of Medicine, Demiroglu Bilim University, Istanbul, Turkey. burcin-aydinozgur@hotmail.com.; Diabetes Application and Research Center, Demiroglu Bilim University, Istanbul, Turkey. burcin-aydinozgur@hotmail.com., Cinar SA; Department of Immunology, Aziz Sancar Institute of Experimental Medicine, Istanbul University, Istanbul, Turkey., Coskunpinar E; Department of Medical Biology, Faculty of Medicine, Health Science University, Istanbul, Turkey., Yilmaz A; Department of Immunology, Aziz Sancar Institute of Experimental Medicine, Istanbul University, Istanbul, Turkey., Altunkanat D; Department of Medical Biology, Faculty of Medicine, Health Science University, Istanbul, Turkey., Deniz G; Department of Immunology, Aziz Sancar Institute of Experimental Medicine, Istanbul University, Istanbul, Turkey., Gurol AO; Department of Immunology, Aziz Sancar Institute of Experimental Medicine, Istanbul University, Istanbul, Turkey.; Diabetes Application and Research Center, Istanbul University, Istanbul, Turkey., Yilmaz MT; Diabetes Application and Research Center, Demiroglu Bilim University, Istanbul, Turkey.; Department of Endocrinology and Metabolism, Faculty of Medicine, Demiroglu Bilim University, Istanbul, Turkey.
Jazyk: angličtina
Zdroj: Immunologic research [Immunol Res] 2023 Jun; Vol. 71 (3), pp. 451-462. Date of Electronic Publication: 2023 Jan 03.
DOI: 10.1007/s12026-022-09355-z
Abstrakt: Th cells play an important role in pathogenesis of type 1 diabetes (T1D). Peripheral blood mononuclear cells were isolated from peripheral blood samples from newly diagnosed (ND), 1-year (1YD), and 5-year T1D (5YD) patients (n:8 of each group), 8 healthy controls (HC), and cultured for 24 h under unstimulated (US) and stimulated conditions. Cell ratios of Th1, Th2, Th17, Treg, and intracellular levels of IFN-γ, TNF-α, IL-10, TGF-β, IL-5, IL-13, IL-17, and IL-21 cytokines were evaluated using the flow cytometry. mRNA expressions of transcription factors T-bet, GATA3, ROR-γt, and FOXP3 of these cells were determined by real-time PCR. Reduced CD4 + CD25 high cell ratios were detected in ND. CD4 + CD25 high cells were found to be reduced in ND and 1YD compared to HC under IL-2-stimulated conditions. Intracellular IFN-γ and TNF-α levels were low in all patients under US and IL-12-stimulated conditions. IL-17A and IL-21 were found to be high in patients with IL-6-stimulated conditions. Expressions of IL-10 and TGF-β have been observed to be reduced in patients. Th1/Th2, Th17/Treg, and Th1/Treg ratios were higher in patient groups. FOXP3 and GATA3 mRNA expressions were found to be low in patients, while RORγt and T-bet mRNA levels were higher than HC. Th1, Th17, and Treg cells and their cytokines have been shown to be associated with type 1 diabetes.
(© 2023. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze: MEDLINE
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